##BLOCKS= 7          
Note:

PROTOCOL REVISION HISTORY:
v1.0.0: original protocol created (MDC)
v1.0.1: 06/30/05 - Updated, spell checked, & formatted to new style guide. (DW)

READER SUITABILITY:
 EMax, VMax, ThermoMax, VersaMax, SpectraMax, SpectraMax Plus, SpectraMax Plus 384, SpectraMax 190, SpectraMax 340PC, SpectraMax 340PC 384, SpectraMax M2, SpectraMax M5
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Note:

Basic Endpoint Protocol

Unfamiliar with SoftMax Pro software?
If you are new to SoftMax Pro software, we strongly recommended spending time familiarizing yourself with the powerful features by completing at least one of the tutorials located in the Tutorials folder.

Protocol Use
Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve.  Modify the instrument setup for the wavelength(s) of interest for your assay.  You may also modify the template to include additional standards, unknowns, and controls.
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Plate:	Plate01	1.3	PlateFormat	Endpoint	Fluorescence	FALSE	Raw	FALSE	1						1	550	1	12	96	510	Automatic	550			3	Automatic			1	8	None	
	Temperature(¡C)	1	2	3	4	5	6	7	8	9	10	11	12		
	23.40	77.342	65.218	99.633	123.136	70.769	76.183	108.332	105.082	67.919	70.262	100.397	49.862		
		71.666	90.929	65.376	65.268	101.57	160.94	40.926	156.754	103.999	100.454	208.876	82.891		
		71.99	72.259	54.022	65.903	46.58	72.511	80.385	114.088	69.75	79.019	90.27	79.04		
		72.379	61.562	67.27	83.572	55.016	55.84	48.277	81.675	73.689	84.848	53.248	126.939		
		78.753	90.129	70.42	131.324	94.41	183.624	41.31	93.093	113.206	290.758	416.747	259.543		
		59.338	91.969	192.737	83.762	124.122	206.676	707.092	239.152	347.563	52.899	86.219	151.323		
		59.781	63.93	53.435	94.944	236.589	116.302	160.41	253.789	222.947	51.971	138.925	176.632		
		62.961	60.136	71.426	82.142	108.782	185.155	131.037	334.31	159.811	72.831	267.299	67.74		
		
~End
Group:	Standards	1
Sample	Conc	BackCalcConc	Wells	Value	MeanValue	SD	CV

Smallest standard value:  

Largest standard value:  

~End
Group:	Unknowns	1
Sample	Wells	Value	R	Result	MeanResult	SD	CV

R - Outside standard range  

~End
Group:	Unk_Dilution	1
Sample	Wells	Value	R	Result	MeanResult	SD	CV	Dilution	AdjResult

R - Outside standard range  

Mean Adjusted Result:  

~End
Group:	Control	1
Sample	Wells	Sample#	Values	MeanValue

~End
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