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UmfdnT\ v*OtC*SzE UE-wy   w		0
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2
d}#k)\   ,b0	9	n	Mz[M_	m8 W  ,NwH:3$b? - aK	<
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nT_3 -g	
V	6Whu2W*CB!ldI?Y&  1n6 		

#Q+a>_NY\v~[    }

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v*_Kl $~
lBxex   +NMv/	8	m			/
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"gM^c7d  #M
(	.		
Mkf wN ' (           i[\	-	
Wj"#%&=}<?+&'(Qq2      	`Q=mBKab'>X~=K))2. )  Gj]	
X$]@,$Rzgfr) 6 
2Rj/=b		


(Y8Dw;o**(    :C:7c?Z}W	    a>L4
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:%.0=%}.5
KPw s( * T   gkB^d	T


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	,Y5GHI^Q+!j^  9mG


A-4{Fh!4 jz M3~#P>"


AZ<MXg<+eV+"c:/ 4F'7V5			'
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a9rT\| 'p$ 7 sk0c	
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),c  [t(8@~-  % R 	W,;IY#8;E
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o  g  2;x!5DNBD(gq' u  	
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?WPiIp)  -;	
(PSaAO]t#i6HV i  Zcd7c_	
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<#OgsO| I *%
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1>v~+ 3		n&/ &/6AZ`7;L)x! + U l_e	
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(Ul6RYkZ    !C9`l


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&B8qTO)* [ Z&+Z	W
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sJDO!Fwl,s0r{X,Dct#0"  5Wu			L
K[bsU](C.  @8&	6	

w:"I{ }qa?h-? l rDG%*3M-k6	P

Qu:&z[K9a%!   (		(
j

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!d"]q$  &   _
<lJZ,		LVi\ p' 5 1X-ai.9<6d	+7CYrv~U|Mm  S 
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m



/4-/P<*E DW]i X iL}4&
J


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Zr%  ViH	


C97QU=EP*_	B 7I
'"	[	=rvICNK :EvGZbi9%/qJ|sN7 k qC2,j5	i

Jtz%y	JF&g`,B" 1  <eB9	
.gz1Lc- \ [ee	@
X
_h`B
Iv	#/lx{    {4F#ro=  	`
~ul = q=f r		_ET 0@S6t5PN _     Ms		
12\q 0E
b
S*  \QzW	
I96HScl?/P* $!	f
'\k[Mlq
; QR	
d	~3  Tg{0Z	a		
?Y3Qg "Xh!7G- @-: dk	
+n$%!t%a	X.*  d '?	{
?Eu pfT/Ha#  ;;	x$[6l@58 XJp	
.X/9G3:R,GjRM3' t}		
-)r0@Wfo,>j}6 e	^

63`O;b 9+bC
I

2J!}nt  X
Y	lez^'(  B;1	
	
BgsDu8p{f zq?RDt<M! /	r	
j~k5GR[k>	O !/2F% c		*t-SF?i 	,N1Id]&| pirCjs bo	q,hi   zTTTN   {TTNT   |TNTA   }NTAG  TCAN  CANG  ANGC  NGCC  TGAN  GANT  ANTC  NTCA  &CGAN  'GANG  (ANGC  )NGCA  /P  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                         &                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              #                                        	                     	                      !                                                                                                                      	            8      
               	      	                  	                     !   
                                             ,   8   0   
   !   2      %             w   \      $            +   R                                                                                                                                                                    &                                                                                                                                                                                                                                                                                                                                                                                                          #              	       	        !                                          	    8  
     	  	      	       ! 
               , 8 0 
 ! 2  %     w \  $    + R   u            5OxP^x5G,-/>F^<9Q(V kZk	g	AA
hD	HHL
Lh(x8<)t%Tevk'   D

9Q~(>X	Z f6'`qPf8q
7	x] P G{Iu(x
5t	
[
	!  `	5	MI	|(B\/;SD		"BSD	"BQcS4ttSD	"cBoPG	:s [J9|e
SPh~B!lbk
>5G9s	p
gGbcCez{oj

 	,hj?{	p
	gG0O>SD		"cB3\"s6|q(Mqu/E5Fnr}5tez1\b]	n	nzP$bo:
wZ YV
c	TgF6mBWf p&5	G9MPX[u`
v#x IS 1Mu1Uu5SD	:"1:BU\   AH0#3)s4Yb9R5/;d&of	.s1G,GpdQespuQs	 Cy
		A	]
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consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="49" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="50" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="51" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="52" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="53" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="54" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="55" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="56" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="57" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="58" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="60" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="61" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="62" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="63" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="64" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="65" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="66" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="67" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="68" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="69" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="70" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="71" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="72" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="73" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="74" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="75" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="76" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="77" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4608-4658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="78" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4608-4658" color="#00e600" type="standard"/></Feature><Feature recentID="79" name="hypothetical protein" directionality="1" translationMW="1929.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="7736" maxFusedRunOn="7736"><Segment range="4608-4658" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="80" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4608-4658" color="#a6acb3" type="standard"/></Feature><Feature recentID="81" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="82" name="FTL_0420" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4444" color="#00e600" type="standard"/></Feature><Feature recentID="83" name="hypothetical protein" directionality="1" translationMW="3690.28" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="90" hitsStopCodon="1"><Segment range="4339-4444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="84" name="tul4 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#a6acb3" type="standard"/></Feature><Feature recentID="85" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4665-4667" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="86" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4665-4667" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="87" name="lacZ" directionality="1" translationMW="89.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7736" maxFusedRunOn="7736"><Segment range="4665-4667" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="88" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4665-4667" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="89" name="potential TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4489" color="#a6acb3" type="standard"/></Feature><Feature recentID="90" name="PRE" directionality="1" type="misc_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4527-4533" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature><Feature recentID="91" name="-35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4540-4545" color="#00ff00" type="standard"/><Q name="note"><V text="-35"/></Q></Feature><Feature recentID="92" name="-10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4563-4568" color="#00ff00" type="standard"/><Q name="note"><V text="-10"/></Q></Feature><Feature recentID="93" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4574" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="94" name="5'UTR pdpA extended" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4574" color="#a6acb3" type="standard"/></Feature></Features>
  4<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4698-4717" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4698-4717" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4698-4717" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4698-4717" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7710-7735" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7710-7735" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7710-7735" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7710-7735" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4901-4922" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4901-4922" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4901-4922" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4901-4922" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5423-5447" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5423-5447" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5423-5447" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5423-5447" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5949-5969" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5949-5969" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5949-5969" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5949-5969" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6471-6493" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6471-6493" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6471-6493" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6471-6493" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6995-7016" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6995-7016" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6995-7016" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6995-7016" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7525-7548" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7525-7548" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7525-7548" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7525-7548" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7711-7742" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7711-7742" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7711-7742" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7711-7742" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="18" name="KROL326  tul4_promoter_construct_r" sequence="atatggtacccgcaaaagctggtaaaagtgttgatt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:30:36Z"><BindingSite location="4332-4363" boundStrand="0" annealedBases="ggtacccgcaaaagctggtaaaagtgttgatt" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="4332-4363" bases="ggtacccgcaaaagctggtaaaagtgttgatt"/></BindingSite><BindingSite simplified="1" location="4332-4363" boundStrand="0" annealedBases="ggtacccgcaaaagctggtaaaagtgttgatt" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="4332-4363" bases="ggtacccgcaaaagctggtaaaagtgttgatt"/></BindingSite></Primer><Primer recentID="19" name="KROL331  pKR71_reverse_frag1" sequence="gtgtattgataaatcgattataacattaattaaaataaaataa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:32:04Z"><BindingSite location="4455-4497" boundStrand="1" annealedBases="gtgtattgataaatcgattataacattaattaaaataaaataa" meltingTemperature="54"><Component hybridizedRange="4455-4497" bases="gtgtattgataaatcgattataacattaattaaaataaaataa"/></BindingSite><BindingSite simplified="1" location="4455-4497" boundStrand="1" annealedBases="gtgtattgataaatcgattataacattaattaaaataaaataa" meltingTemperature="54"><Component hybridizedRange="4455-4497" bases="gtgtattgataaatcgattataacattaattaaaataaaataa"/></BindingSite></Primer><Primer recentID="20" name="KROL332  pKR71_forward_frag2" sequence="taatcgatttatcaatacactttttataactttatcgtc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:34:35Z"><BindingSite location="4478-4516" boundStrand="0" annealedBases="taatcgatttatcaatacactttttataactttatcgtc" meltingTemperature="57"><Component hybridizedRange="4478-4516" bases="taatcgatttatcaatacactttttataactttatcgtc"/></BindingSite><BindingSite simplified="1" location="4478-4516" boundStrand="0" annealedBases="taatcgatttatcaatacactttttataactttatcgtc" meltingTemperature="57"><Component hybridizedRange="4478-4516" bases="taatcgatttatcaatacactttttataactttatcgtc"/></BindingSite></Primer><Primer recentID="21" name="KROL330  pdpA_leader_reverse" sequence="atatGCGGCCGCagttaactgactaattatgtcttgtatat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:35:15Z"><BindingSite location="4629-4665" boundStrand="1" annealedBases="GCGGCCGCagttaactgactaattatgtcttgtatat" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="4629-4665" bases="GCGGCCGCagttaactgactaattatgtcttgtatat"/></BindingSite><BindingSite simplified="1" location="4629-4665" boundStrand="1" annealedBases="GCGGCCGCagttaactgactaattatgtcttgtatat" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="4629-4665" bases="GCGGCCGCagttaactgactaattatgtcttgtatat"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>5fd3e0ab-0979-437a-a8a6-067bff85824c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="15:56:15">2020.10.8</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
  '     "  I   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>TH`Ćxve襅[%N&jY^8j)Ƥ+4FeYGe=,u!)z[>7od?J3O>
7L#S#<g,^)3}r-o<o
)ږ(aSG1i25=K*<Z3j3|084Q0a0گ!˲6WǝմƯb{:jxJш	t@sBL7 -rr_x3oŻ#(^7|s4SB0hU8\5<MжG#Au\3ͦ}	1>& =fp#ˇL\s1[!ೀ&ո?aC}́O<VUX:ᾣs1K¬gC*IʝVa\5ç!g<\l&<|oLMW}4Ҫ*oW~+d\N3SL!H;BWJ<3׸;t#_1`SݴσM ˃_s2o͸}`jlNYL֥U]x8m+#|eh2Rh>0=M24Fw0q26_WH-X1H11o^=83BMWiE^oϧ>*GbgLU8s1hZ`H3zvt^n8E8Y¯f h l0Z3}҂Ue6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          H   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4582-7650" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="21" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7173-7197" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="22" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6529-6553" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="23" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5647-5676" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="24" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-4878" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V 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text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="35" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="36" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7652-8009" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="47" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="48" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="51" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="53" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="54" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="55" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="56" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="57" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="58" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="59" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="60" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="61" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="62" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="63" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="64" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="65" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="66" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="67" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="68" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="69" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="70" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="71" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="72" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="73" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="74" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="75" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="76" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="77" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="78" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="79" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="80" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="81" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="82" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="83" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="84" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="85" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="86" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="87" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4522-4572" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="88" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4522-4572" color="#00e600" type="standard"/></Feature><Feature recentID="89" name="hypothetical protein" directionality="1" translationMW="1929.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="7650" maxFusedRunOn="7650"><Segment range="4522-4572" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="90" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4522-4572" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0420" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4444" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="3690.28" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="90" hitsStopCodon="1"><Segment range="4339-4444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="tul4 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#a6acb3" type="standard"/></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4579-4581" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="11" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4579-4581" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="lacZ" directionality="1" translationMW="89.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7650" maxFusedRunOn="7650"><Segment range="4579-4581" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4579-4581" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature></Features>
  2<?xml version="1.0"?><Primers recycledIDs="1,20,21,22" nextValidID="25"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL323  pdpA_UTR_ext_f" sequence="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4485-4517" boundStrand="0" annealedBases="tttagttatgttctaattaagtagacaatgata" meltingTemperature="52"><Component bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgac"/><Component hybridizedRange="4485-4517" bases="tttagttatgttctaattaagtagacaatgata"/></BindingSite><BindingSite simplified="1" location="4485-4517" boundStrand="0" annealedBases="tttagttatgttctaattaagtagacaatgata" meltingTemperature="52"><Component bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgac"/><Component hybridizedRange="4485-4517" bases="tttagttatgttctaattaagtagacaatgata"/></BindingSite></Primer><Primer recentID="2" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="4" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="5" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="6" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="7" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="8" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="9" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4612-4631" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4612-4631" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4612-4631" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4612-4631" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="12" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7624-7649" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7624-7649" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7624-7649" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7624-7649" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="13" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4815-4836" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4815-4836" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4815-4836" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4815-4836" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="14" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5337-5361" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5337-5361" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5337-5361" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5337-5361" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="15" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5863-5883" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5863-5883" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5863-5883" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5863-5883" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="16" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6385-6407" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6385-6407" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6385-6407" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6385-6407" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="17" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6909-6930" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6909-6930" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6909-6930" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6909-6930" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="18" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7439-7462" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7439-7462" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7439-7462" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7439-7462" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="19" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4575-4599" boundStrand="0" annealedBases="gcGCCATGATTACGGATTCACTGG" meltingTemperature="58"><Component bases="gttctaattaagtagacaatgata"/><Component hybridizedRange="4575-4576" bases="gc"/><Component bases="a"/><Component hybridizedRange="4578-4599" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4575-4599" boundStrand="0" annealedBases="gcGCCATGATTACGGATTCACTGG" meltingTemperature="58"><Component bases="gttctaattaagtagacaatgata"/><Component hybridizedRange="4575-4576" bases="gc"/><Component bases="a"/><Component hybridizedRange="4578-4599" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="23" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7625-7656" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7625-7656" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7625-7656" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7625-7656" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="24" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4493-4521" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4493-4521" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4493-4521" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4493-4521" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>5fd3e0ab-0979-437a-a8a6-067bff85824c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:51:54">2020.8.31</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
  S     !  C   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
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>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>TH`Ćxve襅[%N&jY^8j)Ƥ+4FeYGeiʧ\W2ץvNLQt4X1`Σ}<881Cxэc?nw!iʊݩb89Ps`^*ۈ#\,)t˅~6s<Wϳ`3!JnA(e|y[Lj(a<'1(yJ4W4,|ϫ!Zla2)2.,UW<V[0w78hSE>1
#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S           B   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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recentID="46" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="47" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="48" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="51" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="52" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" 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type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="57" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="58" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="59" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="60" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="61" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="62" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="63" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="64" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="65" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="66" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="67" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="68" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="69" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="70" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="71" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="72" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="73" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="74" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="75" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="76" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="77" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="78" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="79" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="80" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="81" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="82" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="83" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="84" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="85" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="86" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="87" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4522-4572" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="88" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4522-4572" color="#00e600" type="standard"/></Feature><Feature recentID="89" name="hypothetical protein" directionality="1" translationMW="1929.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="7644" maxFusedRunOn="7644"><Segment range="4522-4572" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="90" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4522-4572" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0420" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4444" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="3690.28" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="90" hitsStopCodon="1"><Segment range="4339-4444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="tul4 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#a6acb3" type="standard"/></Feature></Features>
  2<?xml version="1.0"?><Primers recycledIDs="1,20,21,22" nextValidID="25"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL323  pdpA_UTR_ext_f" sequence="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4485-4517" boundStrand="0" annealedBases="tttagttatgttctaattaagtagacaatgata" meltingTemperature="52"><Component bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgac"/><Component hybridizedRange="4485-4517" bases="tttagttatgttctaattaagtagacaatgata"/></BindingSite><BindingSite simplified="1" location="4485-4517" boundStrand="0" annealedBases="tttagttatgttctaattaagtagacaatgata" meltingTemperature="52"><Component bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgac"/><Component hybridizedRange="4485-4517" bases="tttagttatgttctaattaagtagacaatgata"/></BindingSite></Primer><Primer recentID="2" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="4" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="5" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="6" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="7" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="8" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="9" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4606-4625" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4606-4625" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4606-4625" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4606-4625" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="12" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7618-7643" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7618-7643" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7618-7643" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7618-7643" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="13" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4809-4830" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4809-4830" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4809-4830" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4809-4830" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="14" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5331-5355" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5331-5355" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5331-5355" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5331-5355" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="15" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5857-5877" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5857-5877" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5857-5877" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5857-5877" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="16" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6379-6401" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6379-6401" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6379-6401" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6379-6401" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="17" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6903-6924" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6903-6924" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6903-6924" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6903-6924" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="18" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7433-7456" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7433-7456" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7433-7456" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7433-7456" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="19" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4572-4593" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4572-4593" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4572-4593" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4572-4593" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="23" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7619-7650" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7619-7650" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7619-7650" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7619-7650" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="24" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4493-4521" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4493-4521" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4493-4521" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4493-4521" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>5fd3e0ab-0979-437a-a8a6-067bff85824c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:48:14">2020.8.31</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
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type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="74" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="75" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="76" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="77" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="78" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="79" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="80" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="81" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="82" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="83" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="84" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="85" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="86" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="87" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4608-4658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="88" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4608-4658" color="#00e600" type="standard"/></Feature><Feature recentID="89" name="hypothetical protein" directionality="1" translationMW="1929.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="7730" maxFusedRunOn="7730"><Segment range="4608-4658" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="90" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4608-4658" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0420" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4444" color="#00e600" type="standard"/></Feature><Feature recentID="8" name="hypothetical protein" directionality="1" translationMW="3690.28" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="90" hitsStopCodon="1"><Segment range="4339-4444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  2O<?xml version="1.0"?><Primers recycledIDs="1,20,21,22" nextValidID="25"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL323  pdpA_UTR_ext_f" sequence="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4525-4603" boundStrand="0" annealedBases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" meltingTemperature="64"><Component hybridizedRange="4525-4603" bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata"/></BindingSite><BindingSite simplified="1" location="4525-4603" boundStrand="0" annealedBases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" meltingTemperature="64"><Component hybridizedRange="4525-4603" bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata"/></BindingSite></Primer><Primer recentID="2" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="4" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="5" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="6" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="7" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="8" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="9" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4692-4711" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4692-4711" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4692-4711" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4692-4711" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="12" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7704-7729" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7704-7729" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7704-7729" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7704-7729" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="13" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4895-4916" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4895-4916" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4895-4916" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4895-4916" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="14" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5417-5441" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5417-5441" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5417-5441" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5417-5441" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="15" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5943-5963" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5943-5963" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5943-5963" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5943-5963" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="16" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6465-6487" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6465-6487" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6465-6487" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6465-6487" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="17" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6989-7010" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6989-7010" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6989-7010" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6989-7010" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="18" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7519-7542" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7519-7542" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7519-7542" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7519-7542" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="19" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4658-4679" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4658-4679" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4658-4679" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4658-4679" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="23" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7705-7736" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7705-7736" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7705-7736" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7705-7736" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="24" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4579-4607" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4579-4607" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4579-4607" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4579-4607" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>5fd3e0ab-0979-437a-a8a6-067bff85824c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:47:22">2020.8.31</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
  b     0     	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     U4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
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>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFymgzWfKYHV+4FeYGeiʧ\W2ץvNLQt4X1`Σ}<881Cxэc?nw!iʊݩb89Ps`^*ۈ#\,)t˅~6s<Wϳ`3!JnA(e|y[Lj(a<'1(yJ4W4,|ϫ!Zla2)2.,UW<V[0w78hSE>1
#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S           ~   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="79" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="80" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="81" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="82" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="83" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="84" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="85" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="86" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="87" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4582-4632" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="88" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4582-4632" color="#00e600" type="standard"/></Feature><Feature recentID="89" name="hypothetical protein" directionality="1" translationMW="1929.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="7704" maxFusedRunOn="7704"><Segment range="4582-4632" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="90" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4582-4632" color="#a6acb3" type="standard"/></Feature></Features>
  =<?xml version="1.0"?><Primers nextValidID="25"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL323  pdpA_UTR_ext_f" sequence="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4499-4577" boundStrand="0" annealedBases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" meltingTemperature="64"><Component hybridizedRange="4499-4577" bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata"/></BindingSite><BindingSite simplified="1" location="4499-4577" boundStrand="0" annealedBases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" meltingTemperature="64"><Component hybridizedRange="4499-4577" bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata"/></BindingSite></Primer><Primer recentID="1" name="KROL322 pdpA_UTR_ext_r" sequence="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4431-4510" boundStrand="1" annealedBases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag" meltingTemperature="63"><Component hybridizedRange="4431-4510" bases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4510" boundStrand="1" annealedBases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag" meltingTemperature="63"><Component hybridizedRange="4431-4510" bases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="2" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="4" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="5" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="6" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="7" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="8" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="9" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4666-4685" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4666-4685" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4666-4685" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4666-4685" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="12" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7678-7703" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7678-7703" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7678-7703" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7678-7703" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="13" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4869-4890" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4869-4890" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4869-4890" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4869-4890" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="14" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5391-5415" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5391-5415" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5391-5415" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5391-5415" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="15" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5917-5937" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5917-5937" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5917-5937" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5917-5937" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="16" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6439-6461" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6439-6461" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6439-6461" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6439-6461" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="17" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6963-6984" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6963-6984" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6963-6984" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6963-6984" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="18" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7493-7516" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7493-7516" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7493-7516" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7493-7516" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="19" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4632-4653" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4632-4653" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4632-4653" boundStrand="0" annealedBases="GCCATGATTACGGATTCACTGG" meltingTemperature="59"><Component bases="gttctaattaagtagacaatgatagca"/><Component hybridizedRange="4632-4653" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="20" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="21" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="22" name="KROL317  pBfr_construct_r" sequence="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4431-4462" boundStrand="1" annealedBases="ttagtatctaattatatatcaaaatatctaag" meltingTemperature="48"><Component bases="cttaattagaacataac"/><Component hybridizedRange="4431-4462" bases="ttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4462" boundStrand="1" annealedBases="ttagtatctaattatatatcaaaatatctaag" meltingTemperature="48"><Component bases="cttaattagaacataac"/><Component hybridizedRange="4431-4462" bases="ttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="23" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7679-7710" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7679-7710" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7679-7710" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7679-7710" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="24" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4553-4581" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4553-4581" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4553-4581" boundStrand="1" annealedBases="Ctgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGCGGCCG"/><Component hybridizedRange="4553-4581" bases="Ctgctatcattgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>5fd3e0ab-0979-437a-a8a6-067bff85824c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:46:49">2020.8.31</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
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{OIkӄ9J-UHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i          K   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="83" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="84" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="85" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="86" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature></Features>
  ;<?xml version="1.0"?><Primers nextValidID="24"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL323  pdpA_UTR_ext_f" sequence="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4499-4577" boundStrand="0" annealedBases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" meltingTemperature="64"><Component hybridizedRange="4499-4577" bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata"/></BindingSite><BindingSite simplified="1" location="4499-4577" boundStrand="0" annealedBases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata" meltingTemperature="64"><Component hybridizedRange="4499-4577" bases="tagctgtaaacatatagtcaatagataagtgcttatgtaaaatgactttagttatgttctaattaagtagacaatgata"/></BindingSite></Primer><Primer recentID="1" name="KROL322 pdpA_UTR_ext_r" sequence="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4431-4510" boundStrand="1" annealedBases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag" meltingTemperature="63"><Component hybridizedRange="4431-4510" bases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4510" boundStrand="1" annealedBases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag" meltingTemperature="63"><Component hybridizedRange="4431-4510" bases="tgtttacagctaattggtctgacgataaagttataaaaagtgtattgattagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="2" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="3" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="4" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="5" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="6" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="7" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="8" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="9" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="10" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="11" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4615-4634" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4615-4634" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4615-4634" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4615-4634" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="12" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7627-7652" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7627-7652" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7627-7652" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7627-7652" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="13" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4818-4839" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4818-4839" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4818-4839" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4818-4839" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="14" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5340-5364" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5340-5364" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5340-5364" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5340-5364" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="15" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5866-5886" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5866-5886" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5866-5886" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5866-5886" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="16" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6388-6410" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6388-6410" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6388-6410" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6388-6410" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="17" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6912-6933" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6912-6933" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6912-6933" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6912-6933" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="18" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7442-7465" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7442-7465" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7442-7465" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7442-7465" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="19" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4554-4602" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4554-4602" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4554-4602" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4554-4602" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="20" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="21" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="22" name="KROL317  pBfr_construct_r" sequence="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4431-4462" boundStrand="1" annealedBases="ttagtatctaattatatatcaaaatatctaag" meltingTemperature="48"><Component bases="cttaattagaacataac"/><Component hybridizedRange="4431-4462" bases="ttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4462" boundStrand="1" annealedBases="ttagtatctaattatatatcaaaatatctaag" meltingTemperature="48"><Component bases="cttaattagaacataac"/><Component hybridizedRange="4431-4462" bases="ttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="23" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7628-7659" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7628-7659" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7628-7659" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7628-7659" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>5fd3e0ab-0979-437a-a8a6-067bff85824c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="21:42:13">2020.8.28</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
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)ږ(aSG1i25=K*<Z3j3|084Q0a0گ!˲6WǝմƯb{:jxJш	t@sBL7 -rr_x3oŻ#(^7|s4SB0hU8\5<MжG#Au\3ͦ}	1>& =fp#ˇL\s1[!ೀ&ո?aC}́O<VUX:ᾣs1K¬gC*IʝVa\5ç!g<\l&<|oLMW}4Ҫ*oW~+d\N3SL!H;BWJ<3׸;t#_1`SݴσM ˃_s2o͸}`jlNYL֥U]x8m+#|eh2Rh>0=M24Fw0q26_WH-X1H11o^=83BMWiE^oϧ>*GbgLU8s1hZ`H3zvt^n8E8Y¯f h l0Z3}҂Ue6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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maxFusedRunOn="7566"><Segment range="4486-4494" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="74" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4494" color="#a6acb3" type="standard"/></Feature><Feature recentID="75" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4462" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="76" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4462" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="81" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4462" color="#00ccff" type="standard"/></Feature></Features>
  7<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4528-4547" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4528-4547" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4528-4547" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4528-4547" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7540-7565" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7540-7565" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7540-7565" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7540-7565" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4731-4752" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4731-4752" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4731-4752" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4731-4752" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5253-5277" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5253-5277" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5253-5277" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5253-5277" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5779-5799" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5779-5799" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5779-5799" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5779-5799" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6301-6323" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6301-6323" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6301-6323" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6301-6323" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6825-6846" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6825-6846" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6825-6846" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6825-6846" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7355-7378" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7355-7378" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7355-7378" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7355-7378" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4467-4515" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4467-4515" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4467-4515" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4467-4515" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4478" boundStrand="1" annealedBases="cttaattagaacataactagtatctaattatatatcaaaatatctaag" meltingTemperature="51"><Component hybridizedRange="4462-4478" bases="cttaattagaacataac"/><Component bases="t"/><Component hybridizedRange="4431-4461" bases="tagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4478" boundStrand="1" annealedBases="cttaattagaacataactagtatctaattatatatcaaaatatctaag" meltingTemperature="51"><Component hybridizedRange="4462-4478" bases="cttaattagaacataac"/><Component bases="t"/><Component hybridizedRange="4431-4461" bases="tagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7541-7572" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7541-7572" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7541-7572" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7541-7572" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="14:56:11">2020.7.15</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
            	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFyJbiIaOa*rx_^ϣM=82Y1D?7Ҍ`Ń:STH/WF7
q\[*+[{;vTACZj){n p3Aʏ.LکE_>!8)mbL6r죍um1أΥ88Rb0ģ!]*`!\г>0i <lw\W^ L[n
<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"YA퍆l5;LFUC>jSʔUi.NYUs_+սBx]NӖ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  \<?xml version="1.0"?><Features nextValidID="82"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7569-7926" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7569-7597" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7620-7717" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7814-7908" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4486" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4486" color="#a6acb3" type="standard"/></Feature><Feature recentID="61" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7563-7567" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4772-4795" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5564-5593" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6446-6470" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7090-7114" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="66" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4496-7567" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="67" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4496-7567" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="68" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4513-4529" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="69" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4496-7567" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4496-7567" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="71" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4487-4495" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="72" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4487-4495" color="#00e600" type="standard"/></Feature><Feature recentID="73" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7567" maxFusedRunOn="7567"><Segment range="4487-4495" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="74" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4487-4495" color="#a6acb3" type="standard"/></Feature><Feature recentID="75" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="76" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="77" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="78" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="81" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  6<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4529-4548" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4529-4548" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4529-4548" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4529-4548" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7541-7566" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7541-7566" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7541-7566" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7541-7566" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4732-4753" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4732-4753" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4732-4753" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4732-4753" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5254-5278" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5254-5278" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5254-5278" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5254-5278" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5780-5800" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5780-5800" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5780-5800" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5780-5800" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6302-6324" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6302-6324" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6302-6324" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6302-6324" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6826-6847" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6826-6847" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6826-6847" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6826-6847" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7356-7379" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7356-7379" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7356-7379" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7356-7379" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4468-4516" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4468-4516" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4468-4516" boundStrand="0" annealedBases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4468-4516" bases="gttctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4479" boundStrand="1" annealedBases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="cttaattagaacataacttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7542-7573" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7542-7573" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7542-7573" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7542-7573" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="14:55:49">2020.7.15</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
            	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFyRZRGXdiʧ\W2ץvNLQt4X1`Σ}<881Cxэc?nw!iʊݩb89Ps`^*ۈ#\,)t˅~6s<Wϳ`3!JnA(e|y[Lj(a<'1(yJ4W4,|ϫ!Zla2)2.,UW<V[0w78hSE>1
#X.8S8k_-o-`a4q48aM^#8o #9bShW=al|a|1̗1`3%"n8amp[qlm[&0<w=j?Q#`U91g?s	t*Vx420. m,fU_,:psJap.6wM*J5}H}1g⼶Le`8%<%tx^|<{I̒5s6E<L40UH;/ۇLV6%j_=-шeU׎ҷ7\v%!L6s,\P}(H4gz8c;s m Ujm<~|$Ճ|6Ӄ4$0%x;<U|x|hv&˯\.330ǯJm<K]5_OlP݃*`q?-&N<,}(.VPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S              -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ]<?xml version="1.0"?><Features nextValidID="83"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7927" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7598" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7621-7718" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7815-7909" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4464" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="61" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7568" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4773-4796" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5565-5594" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6447-6471" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="66" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7091-7115" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="67" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="68" name="lacZ" directionality="1" translationMW="116321.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" hitsStopCodon="1"><Segment range="4497-7568" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="69" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4514-4530" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7568" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="72" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="73" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#00e600" type="standard"/></Feature><Feature recentID="74" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7568" maxFusedRunOn="7568"><Segment range="4488-4496" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="75" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#a6acb3" type="standard"/></Feature><Feature recentID="76" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="77" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="81" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="82" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  7<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4530-4549" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4530-4549" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4530-4549" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4530-4549" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7542-7567" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7542-7567" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7542-7567" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7542-7567" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4733-4754" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4733-4754" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4733-4754" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4733-4754" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5255-5279" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5255-5279" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5255-5279" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5255-5279" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5781-5801" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5781-5801" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5781-5801" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5781-5801" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6303-6325" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6303-6325" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6303-6325" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6303-6325" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6827-6848" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6827-6848" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6827-6848" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6827-6848" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7357-7380" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7357-7380" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7357-7380" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7357-7380" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4472-4517" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="62"><Component hybridizedRange="4472-4495" bases="ctaattaagtagacaatgatagca"/><Component bases="ATG"/><Component hybridizedRange="4496-4517" bases="GCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4472-4517" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaGCCATGATTACGGATTCACTGG" meltingTemperature="62"><Component hybridizedRange="4472-4495" bases="ctaattaagtagacaatgatagca"/><Component bases="ATG"/><Component hybridizedRange="4496-4517" bases="GCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7543-7574" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7543-7574" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7543-7574" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7543-7574" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="18:1:53">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  8          	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>YUdznUdfکY
FjfZFyRZRGXda*rx_^ϣM=82Y1D?7Ҍ`Ń:STH/WF7
q\[*+[{;vTACZj){n p3Aʏ.LکE_>!8)mbL6r죍um1أΥ88Rb0ģ!]*`!\г>0i <lw\W^ L[n
<pM,*`O(C)|m`-f4]z<i_o8w8| aOY_6892\ł4lĈm,=9m3n,o4Ppibs3 aG8VDs?8oŜ`%09Yʲo@ìaW j=+ຏ7<8pSUM4+ʛ!ş17`SR>bP҆yOL5'2H|-7m3H2W"̿sn}1XS(5|:F h1W^8s3J8q1πsCi<SL#pѝ#|̃MW驴Uh`Vi(RLg|0h>NГD@QWϊn=ؙ'.p0Z?w+]-t׆}=ێ70QCvlpـ1Ǿ8Li"YA퍆l5;LFUC>jSʔUi.NYUs_+սBx]NӖ             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ]<?xml version="1.0"?><Features nextValidID="83"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7573-7930" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7573-7601" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7624-7721" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7818-7912" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4464" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="61" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4464-4487" color="#a6acb3" type="standard"/></Feature><Feature recentID="62" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-7571" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="63" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4776-4799" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="64" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5568-5597" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="65" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6450-6474" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="66" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7094-7118" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="67" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7571" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="68" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4497-7571" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="69" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4517-4533" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="70" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7571" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4497-7571" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="72" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="73" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#00e600" type="standard"/></Feature><Feature recentID="74" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7571" maxFusedRunOn="7571"><Segment range="4488-4496" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="75" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4488-4496" color="#a6acb3" type="standard"/></Feature><Feature recentID="76" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="77" name="P296 Pbfr_R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4460-4463" color="#8080ff" type="standard"/><Q name="note"><V text="P296 Pbfr_R"/></Q></Feature><Feature recentID="78" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4463-4463" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="79" name="Untitled" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4449-4455" color="#00ff00" type="standard"/></Feature><Feature recentID="80" name="Untitled" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4425-4430" color="#00ff00" type="standard"/></Feature><Feature recentID="81" name="P574 Pbfr_1F" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4373-4403" color="#50ff50" type="standard"/><Q name="note"><V text="P574 Pbfr_1F"/></Q></Feature><Feature recentID="82" name="Bfr promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4463" color="#00ccff" type="standard"/></Feature></Features>
  6<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4533-4552" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4533-4552" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4533-4552" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4533-4552" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7545-7570" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7545-7570" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7545-7570" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7545-7570" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4736-4757" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4736-4757" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4736-4757" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4736-4757" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5258-5282" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5258-5282" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5258-5282" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5258-5282" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5784-5804" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5784-5804" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5784-5804" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5784-5804" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6306-6328" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6306-6328" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6306-6328" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6306-6328" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6830-6851" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6830-6851" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6830-6851" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6830-6851" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7360-7383" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7360-7383" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7360-7383" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7360-7383" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL318  LacZ_construct_f" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4472-4520" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4472-4520" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4472-4520" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4472-4520" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="18" name="P574 Pbfr_1F" sequence="ATATGGATCCaatttaacttatctaaaaatagcataatatc" description="Contains BamHI site"><BindingSite location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite><BindingSite simplified="1" location="4372-4402" boundStrand="0" annealedBases="aatttaacttatctaaaaatagcataatatc" meltingTemperature="50"><Component bases="ATATGGATCC"/><Component hybridizedRange="4372-4402" bases="aatttaacttatctaaaaatagcataatatc"/></BindingSite></Primer><Primer recentID="19" name="KROL316 pBfr_construct_f" sequence="atatggtaccttatttttcttaaatattattattaaaaatagta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:37:17Z"><BindingSite location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite><BindingSite simplified="1" location="4332-4371" boundStrand="0" annealedBases="ggtaccttatttttcttaaatattattattaaaaatagta" meltingTemperature="52"><Component bases="atat"/><Component hybridizedRange="4332-4371" bases="ggtaccttatttttcttaaatattattattaaaaatagta"/></BindingSite></Primer><Primer recentID="20" name="KROL317  pBfr_construct_r" sequence="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:40:29Z"><BindingSite location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite><BindingSite simplified="1" location="4431-4479" boundStrand="1" annealedBases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag" meltingTemperature="55"><Component hybridizedRange="4431-4479" bases="ttaattagaacataactttagtatctaattatatatcaaaatatctaag"/></BindingSite></Primer><Primer recentID="21" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-23T14:43:19Z"><BindingSite location="7546-7577" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7546-7577" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7546-7577" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7546-7577" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="18:1:45">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     S4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>JbiIaX=,u!)z[>7od?J3O>
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)ږ(aSG1i25=K*<Z3j3|084Q0a0گ!˲6WǝմƯb{:jxJш	t@sBL7 -rr_x3oŻ#(^7|s4SB0hU8\5<MжG#Au\3ͦ}	1>& =fp#ˇL\s1[!ೀ&ո?aC}́O<VUX:ᾣs1K¬gC*IʝVa\5ç!g<\l&<|oLMW}4Ҫ*oW~+d\N3SL!H;BWJ<3׸;t#_1`SݴσM ˃_s2o͸}`jlNYL֥U]x8m+#|eh2Rh>0=M24Fw0q26_WH-X1H11o^=83BMWiE^oϧ>*GbgLU8s1hZ`H3zvt^n8E8Y¯f h l0Z3}҂Ue6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  W<?xml version="1.0"?><Features recycledIDs="59,60,61,62,63,64,65" nextValidID="83"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7448-7805" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7448-7476" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7499-7596" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7693-7787" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="66" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4362" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="67" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4339" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="68" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4362" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7442-7446" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="70" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4651-4674" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="71" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5443-5472" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="72" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6325-6349" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="73" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6969-6993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="74" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4372-7446" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="75" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4372-7446" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="76" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4392-4408" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="77" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4372-7446" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="78" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4372-7446" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="79" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4363-4371" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="80" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4363-4371" color="#00e600" type="standard"/></Feature><Feature recentID="81" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7446" maxFusedRunOn="7446"><Segment range="4363-4371" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="82" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4363-4371" color="#a6acb3" type="standard"/></Feature></Features>
  1r<?xml version="1.0"?><Primers recycledIDs="7,8,9,10,13,14,15" nextValidID="26"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="11" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="12" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="16" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4408-4427" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4408-4427" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4408-4427" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4408-4427" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="17" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7420-7445" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7420-7445" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7420-7445" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7420-7445" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="18" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4611-4632" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4611-4632" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4611-4632" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4611-4632" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="19" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5133-5157" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5133-5157" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5133-5157" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5133-5157" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="20" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5659-5679" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5659-5679" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5659-5679" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5659-5679" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="21" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6181-6203" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6181-6203" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6181-6203" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6181-6203" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="22" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6705-6726" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6705-6726" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6705-6726" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6705-6726" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="23" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7235-7258" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7235-7258" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7235-7258" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7235-7258" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="24" name="Primer XXXX f2" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4347-4395" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4347-4395" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4347-4395" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4347-4395" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="25" name="Primer XXX r1" sequence="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T21:48:04Z"><BindingSite location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite><BindingSite simplified="1" location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite simplified="1" location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="2:36:3">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
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-&*t:ˀxK8iYp01qҸ\.sͽ35]JҼ_lRLY-q;x 	O850#,u	](h^>4D^s$|\͂Ov>4.~!<=68	e2ZKCtbUu<1HS͡\80C4(7ގ8i4@~O_	 `Ɵ(b wͼ3x	4L	^5y><8-r1Wši} 1ҵO9Mx`W=s7`f
{OIkӄ9J-UHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i             -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ]<?xml version="1.0"?><Features nextValidID="83"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7595-7952" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7595-7623" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7646-7743" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7840-7934" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4485" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="61" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4485" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="62" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4485" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="63" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="64" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="65" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="66" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4509" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="67" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4486" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="68" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4486-4509" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7589-7593" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="70" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4798-4821" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="71" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5590-5619" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="72" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6472-6496" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="73" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7116-7140" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="74" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4519-7593" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="75" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4519-7593" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="76" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4539-4555" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="77" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4519-7593" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="78" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4519-7593" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="79" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4518" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="80" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4510-4518" color="#00e600" type="standard"/></Feature><Feature recentID="81" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7593" maxFusedRunOn="7593"><Segment range="4510-4518" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="82" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4518" color="#a6acb3" type="standard"/></Feature></Features>
  K|<?xml version="1.0"?><Primers nextValidID="26"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="8" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="9" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="10" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="11" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="12" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="13" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="16" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4555-4574" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4555-4574" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4555-4574" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4555-4574" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="17" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7567-7592" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7567-7592" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7567-7592" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7567-7592" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="18" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4758-4779" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4758-4779" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4758-4779" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4758-4779" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="19" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5280-5304" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5280-5304" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5280-5304" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5280-5304" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="20" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5806-5826" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5806-5826" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5806-5826" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5806-5826" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="21" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6328-6350" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6328-6350" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6328-6350" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6328-6350" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="22" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6852-6873" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6852-6873" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6852-6873" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6852-6873" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="23" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7382-7405" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7382-7405" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7382-7405" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7382-7405" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="24" name="Primer XXXX f2" sequence="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4494-4542" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4494-4542" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4494-4542" boundStrand="0" annealedBases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4494-4542" bases="ctaattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="25" name="Primer XXX r1" sequence="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T21:48:04Z"><BindingSite location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite><BindingSite location="4454-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="58"><Component hybridizedRange="4454-4502" bases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite location="4455-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaattt" meltingTemperature="52"><Component hybridizedRange="4465-4502" bases="cttaattagaacataactagtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="4459-4464" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="4455-4457" bases="ttt"/></BindingSite><BindingSite simplified="1" location="186-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="54"><Component bases="cttaattagaacataact"/><Component hybridizedRange="186-216" bases="agtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite simplified="1" location="187-216" boundStrand="1" annealedBases="agtctagatattgagtcaaaaaaaaattt" meltingTemperature="43"><Component bases="cttaattagaacataact"/><Component hybridizedRange="197-216" bases="agtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="191-196" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="187-189" bases="ttt"/></BindingSite><BindingSite simplified="1" location="4454-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt" meltingTemperature="58"><Component hybridizedRange="4454-4502" bases="cttaattagaacataactagtctagatattgagtcaaaaaaaaaatttt"/></BindingSite><BindingSite simplified="1" location="4455-4502" boundStrand="1" annealedBases="cttaattagaacataactagtctagatattgagtcaaaaaaaaattt" meltingTemperature="52"><Component hybridizedRange="4465-4502" bases="cttaattagaacataactagtctagatattgagtcaaa"/><Component bases="a"/><Component hybridizedRange="4459-4464" bases="aaaaaa"/><Component bases="t"/><Component hybridizedRange="4455-4457" bases="ttt"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="2:35:51">2020.1.23</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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)ږ(aSG1i25=K*<Z3j3|084Q0a0گ!˲6WǝմƯb{:jxJш	t@sBL7 -rr_x3oŻ#(^7|s4SB0hU8\5<MжG#Au\3ͦ}	1>& =fp#ˇL\s1[!ೀ&ո?aC}́O<VUX:ᾣs1K¬gC*IʝVa\5ç!g<\l&<|oLMW}4Ҫ*oW~+d\N3SL!H;BWJ<3׸;t#_1`SݴσM ˃_s2o͸}`jlNYL֥U]x8m+#|eh2Rh>0=M24Fw0q26_WH-X1H11o^=83BMWiE^oϧ>*GbgLU8s1hZ`H3zvt^n8E8Y¯f h l0Z3}҂Ue6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      .  3     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  c<?xml version="1.0"?><Features nextValidID="87"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="8" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="17" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7732-8089" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7732-7760" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7783-7880" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7977-8071" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4577-4622" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="co-chaperonin GroES" directionality="1" translationMW="1837.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4639" maxFusedRunOn="4639"><Segment range="4577-4622" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="63" name="P051 groESpromoR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4586" color="#0080ff" type="standard"/><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="64" name="P225 SP_p1715_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4588-4614" color="#8080ff" type="standard"/><Q name="note"><V text="P225 SP_p1715_R"/></Q></Feature><Feature recentID="65" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4521" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="66" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="67" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="68" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="70" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4623" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="72" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature><Feature recentID="73" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7726-7730" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="74" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4935-4958" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="75" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5727-5756" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="76" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6609-6633" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="77" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7253-7277" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="78" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4656-7730" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="79" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4656-7730" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="80" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4676-4692" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="81" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4656-7730" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="82" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4656-7730" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="83" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4647-4655" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="84" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4647-4655" color="#00e600" type="standard"/></Feature><Feature recentID="85" name="hypothetical protein" directionality="1" translationMW="333.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7730" maxFusedRunOn="7730"><Segment range="4647-4655" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="86" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4647-4655" color="#a6acb3" type="standard"/></Feature></Features>
  S<?xml version="1.0"?><Primers nextValidID="28"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="8" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="9" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="10" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="11" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="12" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="13" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="14" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="16" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="17" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4660" boundStrand="1" annealedBases="GGCCtgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGC"/><Component hybridizedRange="4657-4660" bases="GGCC"/><Component bases="GC"/><Component hybridizedRange="4627-4654" bases="tgctatcattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4627-4660" boundStrand="1" annealedBases="GGCCtgctatcattgtctacttaattagaaca" meltingTemperature="55"><Component bases="atatGC"/><Component hybridizedRange="4657-4660" bases="GGCC"/><Component bases="GC"/><Component hybridizedRange="4627-4654" bases="tgctatcattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="18" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4692-4711" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4692-4711" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4692-4711" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4692-4711" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="19" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7704-7729" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7704-7729" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7704-7729" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7704-7729" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="20" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4895-4916" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4895-4916" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4895-4916" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4895-4916" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="21" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5417-5441" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5417-5441" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5417-5441" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5417-5441" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="22" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5943-5963" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5943-5963" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5943-5963" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5943-5963" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="23" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6465-6487" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6465-6487" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6465-6487" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6465-6487" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="24" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6989-7010" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6989-7010" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6989-7010" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6989-7010" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="25" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7519-7542" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7519-7542" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7519-7542" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7519-7542" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="26" name="Primer XXXX f2" sequence="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T20:58:49Z"><BindingSite location="4632-4679" boundStrand="0" annealedBases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4632-4679" bases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite><BindingSite simplified="1" location="4632-4679" boundStrand="0" annealedBases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG" meltingTemperature="66"><Component hybridizedRange="4632-4679" bases="taattaagtagacaatgatagcaATGGCCATGATTACGGATTCACTGG"/></BindingSite></Primer><Primer recentID="27" name="Primer XXXX r1" sequence="gtctacttaattagaacataactctgcacgacgaactaatactctatct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-01-22T21:00:15Z"><BindingSite location="4596-4644" boundStrand="1" annealedBases="gtctacttaattagaacataactctgcacgacgaactaatactctatct" meltingTemperature="64"><Component hybridizedRange="4596-4644" bases="gtctacttaattagaacataactctgcacgacgaactaatactctatct"/></BindingSite><BindingSite simplified="1" location="4596-4644" boundStrand="1" annealedBases="gtctacttaattagaacataactctgcacgacgaactaatactctatct" meltingTemperature="64"><Component hybridizedRange="4596-4644" bases="gtctacttaattagaacataactctgcacgacgaactaatactctatct"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="21:45:9">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk/idI4JbiI`pkx<,Ul4ݿӃ%C|l](>X3*E0Nn,x4cp۝~oppEp(jXNt=Ŧ2ʶ8
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{OIkӄ9J-UHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i      %  *     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7723-7751" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning 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allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="70" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4623" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="72" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature><Feature recentID="73" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7717-7721" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="RPp7"/></Q></Feature><Feature recentID="74" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4926-4949" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS3"/></Q></Feature><Feature recentID="75" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5718-5747" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS4"/></Q></Feature><Feature recentID="76" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6600-6624" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBSK_FS5"/></Q></Feature><Feature recentID="77" name="PCR_primer" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7244-7268" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="MOOL_pBKS_FS6"/></Q></Feature><Feature recentID="78" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4647-7721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="79" name="lacZ" directionality="1" translationMW="116453.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4647-7721" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="80" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4667-4683" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="81" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4647-7721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="82" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4647-7721" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature></Features>
  Mc<?xml version="1.0"?><Primers nextValidID="26"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="11" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="1" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="3" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="4" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="5" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="6" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="13" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4648" boundStrand="1" annealedBases="cattgtctacttaattagaaca" meltingTemperature="48"><Component bases="atatGCGGCCGCtgctat"/><Component hybridizedRange="4627-4648" bases="cattgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4627-4648" boundStrand="1" annealedBases="cattgtctacttaattagaaca" meltingTemperature="48"><Component bases="atatGCGGCCGCtgctat"/><Component hybridizedRange="4627-4648" bases="cattgtctacttaattagaaca"/></BindingSite></Primer><Primer recentID="16" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4683-4702" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4683-4702" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4683-4702" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4683-4702" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="17" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T14:21:49Z"><BindingSite location="7695-7720" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7695-7720" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7695-7720" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7695-7720" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="12" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:21:59Z"><BindingSite location="4886-4907" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4886-4907" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4886-4907" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4886-4907" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="0" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:24:04Z"><BindingSite location="5408-5432" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5408-5432" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5408-5432" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5408-5432" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="22" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:26:51Z"><BindingSite location="5934-5954" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5934-5954" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5934-5954" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5934-5954" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="23" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:28:06Z"><BindingSite location="6456-6478" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6456-6478" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6456-6478" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6456-6478" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="24" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:29:23Z"><BindingSite location="6980-7001" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6980-7001" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6980-7001" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6980-7001" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="25" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-05-21T19:31:41Z"><BindingSite location="7510-7533" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7510-7533" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7510-7533" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7510-7533" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:42:56">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       3     	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     c4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk/idI4JbiI6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      %  '     -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4577-4622" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="co-chaperonin GroES" directionality="1" translationMW="1837.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4639" maxFusedRunOn="4639"><Segment range="4577-4622" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="63" name="P051 groESpromoR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4586" color="#0080ff" type="standard"/><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="64" name="P225 SP_p1715_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4588-4614" color="#8080ff" type="standard"/><Q name="note"><V text="P225 SP_p1715_R"/></Q></Feature><Feature recentID="65" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4521" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="66" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="67" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="68" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature><Feature recentID="70" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="71" name="TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4623" color="#0080ff" type="standard"/><Q name="note"><V text="TSS"/></Q></Feature><Feature recentID="72" name="5'UTR pdpA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4623-4646" color="#a6acb3" type="standard"/></Feature></Features>
  :<?xml version="1.0"?><Primers recycledIDs="16,17,12,0" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="11" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="1" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="3" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="4" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="5" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="6" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="13" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="15" name="P823 DFPI_FR1R2" sequence="atatGCGGCCGCtgctatcattgtctacttaattagaaca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4627-4687" boundStrand="1" annealedBases="tGCGGCCtatcattgtctacttaattagaaca" meltingTemperature="44"><Component bases="ata"/><Component hybridizedRange="4681-4687" bases="tGCGGCC"/><Component bases="GCtgc"/><Component hybridizedRange="4668-4673" bases="tatcat"/><Component hybridizedRange="4627-4645" bases="tgtctacttaattagaaca"/></BindingSite><BindingSite simplified="1" location="4627-4645" boundStrand="1" annealedBases="tgtctacttaattagaaca" meltingTemperature="44"><Component bases="atatGCGGCCGCtgctatcat"/><Component hybridizedRange="4627-4645" bases="tgtctacttaattagaaca"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:41:25">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       -  u   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     K4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:VW䪩iRlDuUuUjUfz5^+cgVX]Uhk/idI46<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          t   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  N<?xml version="1.0"?><Features nextValidID="70"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="4" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="5" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="6" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="7" name="(null)" type="misc_feature" allowSegmentOverlaps="0" 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name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="22" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="23" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="24" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" 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range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4624-4981" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4624-4652" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4675-4772" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4869-4963" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="60" name="groES" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4577-4622" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="co-chaperonin GroES" directionality="1" translationMW="1837.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4633" maxFusedRunOn="4633"><Segment range="4577-4622" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="62" name="P052 groESpromoF" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4366" color="#0080ff" type="standard"/><Q name="note"><V text="P052 groESpromoF"/></Q></Feature><Feature recentID="63" name="P051 groESpromoR" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4586" color="#0080ff" type="standard"/><Q name="note"><V text="P051 groESpromoR"/></Q></Feature><Feature recentID="64" name="P225 SP_p1715_R" directionality="1" type="primer_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4588-4614" color="#8080ff" type="standard"/><Q name="note"><V text="P225 SP_p1715_R"/></Q></Feature><Feature recentID="65" name="pF2 promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4451-4521" color="#0080ff" type="standard"/><Q name="note"><V text="pF2 promoter"/></Q></Feature><Feature recentID="66" name="pF promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4339-4622" color="#0080ff" type="standard"/><Q name="note"><V text="pF promoter"/></Q></Feature><Feature recentID="67" name="GroES -35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4452-4457" color="#a6acb3" type="standard"/></Feature><Feature recentID="68" name="GroES -10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4473-4478" color="#a6acb3" type="standard"/></Feature><Feature recentID="69" name="TSS" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4485-4485" color="#ffffff" type="standard"/></Feature></Features>
  7<?xml version="1.0"?><Primers recycledIDs="15,16,17,12,0" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="4487-4512" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="4487-4512" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="4476-4500" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="4476-4500" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="11" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-04-01T15:45:25Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="4500-4526" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="4500-4526" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="1" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="4408-4434" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="4408-4434" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="P225 SP_p1715_R" sequence="tccattacaagatagagtattagttcg" description="0"><BindingSite location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite><BindingSite simplified="1" location="4587-4613" boundStrand="0" annealedBases="tccattacaagatagagtattagttcg" meltingTemperature="55"><Component hybridizedRange="4587-4613" bases="tccattacaagatagagtattagttcg"/></BindingSite></Primer><Primer recentID="3" name="P457 S_pSD26_1" sequence="ttaagtttatttttcaaagcttgaaggg" description="0"><BindingSite location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite><BindingSite simplified="1" location="4342-4369" boundStrand="1" annealedBases="ttaagtttatttttcaaagcttgaaggg" meltingTemperature="56"><Component hybridizedRange="4342-4369" bases="ttaagtttatttttcaaagcttgaaggg"/></BindingSite></Primer><Primer recentID="4" name="P572 Tn_Mar3" sequence="attaagtttatttttcaaagcttgaagg" description="0"><BindingSite location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite><BindingSite simplified="1" location="4343-4370" boundStrand="1" annealedBases="attaagtttatttttcaaagcttgaagg" meltingTemperature="54"><Component hybridizedRange="4343-4370" bases="attaagtttatttttcaaagcttgaagg"/></BindingSite></Primer><Primer recentID="5" name="P711 KanCass_F" sequence="atatCATATGtatacccttcaagctttgaaaaataaac" description="Contains NdeI site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCATATG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="6" name="P506 KanCass_F" sequence="atatCTCGAGtatacccttcaagctttgaaaaataaac" description="Contains XhoI site"><BindingSite location="4329-4365" boundStrand="0" annealedBases="CGAGtatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCT"/><Component hybridizedRange="4329-4332" bases="CGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatCTCGAG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4585" boundStrand="1" annealedBases="gaatgttcataacaatcttactcctttg" meltingTemperature="55"><Component bases="atatCTGCAG"/><Component hybridizedRange="4558-4585" bases="gaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite location="4558-4591" boundStrand="1" annealedBases="atGGACgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="at"/><Component hybridizedRange="4587-4591" bases="atGGA"/><Component bases="TC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="4558-4586" boundStrand="1" annealedBases="Cgaatgttcataacaatcttactcctttg" meltingTemperature="56"><Component bases="atatGGATC"/><Component hybridizedRange="4558-4586" bases="Cgaatgttcataacaatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="P052 groESpromoF" sequence="atatCTGCAGatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F PstI; F. tularensis LVS"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatCTGCAGa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="13" name="P050 groESpromoR" sequence="atatGGATCCatatacccttcaagctttgaaaaataaac" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="55"><Component bases="atatGGATCCa"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer><Primer recentID="14" name="P508 KanCass_F" sequence="atatGATATCCAATTGtatacccttcaagctttgaaaaataaac" description="&quot;Contains EcoRV site, MfeI site&quot;"><BindingSite location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite><BindingSite simplified="1" location="4338-4365" boundStrand="0" annealedBases="tatacccttcaagctttgaaaaataaac" meltingTemperature="53"><Component bases="atatGATATCCAATTG"/><Component hybridizedRange="4338-4365" bases="tatacccttcaagctttgaaaaataaac"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:38:57">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  U       Y   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     /4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ          X   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  <?xml version="1.0"?><Primers recycledIDs="11,12,0,1,2,3,4,5,6,7,8,9,13,14,15,16,17" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:37:13">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  V       \   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     24~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>HNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i          [   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious 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type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4343-4700" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" 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consecutiveTranslationNumbering="1"><Segment range="4588-4682" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4339-4341" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="11,12,0,1,2,3,4,5,6,7,8,9,13,14,15,16,17" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  a<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:35:0">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  p   
  k  K   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     !4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61D	QeU~fUSBm]EiWtQY9UN99UE5.<ٞXYBVPoy!;ca/N0{A+|uuѕqP.xO*>D2}eUZs=KkU\0uoFA/S               J   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4838-5195" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4838-4866" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4889-4986" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5083-5177" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4593" color="#00ff00" type="standard"/></Feature><Feature recentID="60" name="30S ribosomal protein S21" directionality="1" translationMW="7816.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4836" maxFusedRunOn="4836"><Segment range="4594-4791" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="61" name="rpsU3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4594-4791" color="#00e600" type="standard"/></Feature><Feature recentID="62" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4594-4791" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="63" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4801-4833" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4594-4836" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  2R<?xml version="1.0"?><Primers recycledIDs="11,12" nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL42 OE_rpsU3R" sequence="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4762-4802" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4762-4802" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="2" name="KROL41 OE_rpsU3F" sequence="atatGAATTCaggagggataatatgccaagaataatagttgacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4587-4614" boundStrand="0" annealedBases="gagggtatgccaagaataatagttgacc" meltingTemperature="52"><Component bases="atatGAATTCag"/><Component hybridizedRange="4587-4591" bases="gaggg"/><Component bases="ataa"/><Component hybridizedRange="4592-4614" bases="tatgccaagaataatagttgacc"/></BindingSite><BindingSite simplified="1" location="4592-4614" boundStrand="0" annealedBases="tatgccaagaataatagttgacc" meltingTemperature="52"><Component bases="atatGAATTCaggagggataa"/><Component hybridizedRange="4592-4614" bases="tatgccaagaataatagttgacc"/></BindingSite></Primer><Primer recentID="3" name="KROL290 rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" dateAdded="2019-12-19T21:43:09Z"><BindingSite location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="4" name="KROL291 VSVG_bamHI_r" sequence="atatggatcCTTATTTACCTAATCTATTCATT" dateAdded="2019-12-19T21:43:09Z"><BindingSite location="4814-4842" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="4814-4842" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite><BindingSite simplified="1" location="4814-4842" boundStrand="1" annealedBases="tggatcCTTATTTACCTAATCTATTCATT" meltingTemperature="55"><Component bases="ata"/><Component hybridizedRange="4814-4842" bases="tggatcCTTATTTACCTAATCTATTCATT"/></BindingSite></Primer><Primer recentID="5" name="KROL296 rpsU3_p2_F" sequence="gtgagagggtatgccaagaataatagttgaccctaaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:44:40Z"><BindingSite location="4583-4619" boundStrand="0" annealedBases="gtgagagggtatgccaagaataatagttgaccctaaa" meltingTemperature="63"><Component hybridizedRange="4583-4619" bases="gtgagagggtatgccaagaataatagttgaccctaaa"/></BindingSite><BindingSite simplified="1" location="4583-4619" boundStrand="0" annealedBases="gtgagagggtatgccaagaataatagttgaccctaaa" meltingTemperature="63"><Component hybridizedRange="4583-4619" bases="gtgagagggtatgccaagaataatagttgaccctaaa"/></BindingSite></Primer><Primer recentID="6" name="KROL297 rpsU3_p2_R" sequence="ttcttggcataccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T21:45:37Z"><BindingSite location="4569-4602" boundStrand="1" annealedBases="ttcttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component hybridizedRange="4569-4602" bases="ttcttggcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4602" boundStrand="1" annealedBases="ttcttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component hybridizedRange="4569-4602" bases="ttcttggcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="7" name="KROL263 pKR38_FR1R" sequence="atatgcggccgcctaataagaataagaccttcttgcttcttttttag" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4759-4802" boundStrand="1" annealedBases="atatgcggccgcataagaataagaccttcttgcttcttttttag" meltingTemperature="62"><Component hybridizedRange="4791-4802" bases="atatgcggccgc"/><Component bases="cta"/><Component hybridizedRange="4759-4790" bases="ataagaataagaccttcttgcttcttttttag"/></BindingSite><BindingSite simplified="1" location="4759-4802" boundStrand="1" annealedBases="atatgcggccgcataagaataagaccttcttgcttcttttttag" meltingTemperature="62"><Component hybridizedRange="4791-4802" bases="atatgcggccgc"/><Component bases="cta"/><Component hybridizedRange="4759-4790" bases="ataagaataagaccttcttgcttcttttttag"/></BindingSite></Primer><Primer recentID="8" name="KROL42 OE_rpsU3Ra" sequence="atatGCGGCCGCataagaataagaccttcttgcttcttttt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4762-4802" boundStrand="1" annealedBases="atatGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="atatGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4762-4802" boundStrand="1" annealedBases="atatGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4762-4802" bases="atatGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="9" name="KROL214 ChaC-V_FR1_R" sequence="atatCAATTGaattcttatttacctaatctattcatt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4814-4836" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="4814-4836" bases="cttatttacctaatctattcatt"/></BindingSite><BindingSite simplified="1" location="4814-4836" boundStrand="1" annealedBases="cttatttacctaatctattcatt" meltingTemperature="46"><Component bases="atatCAATTGaatt"/><Component hybridizedRange="4814-4836" bases="cttatttacctaatctattcatt"/></BindingSite></Primer><Primer recentID="10" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="13" name="KROL120 ?rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4579-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcg" meltingTemperature="62"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="4579-4600" bases="cttggcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="4579-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcg" meltingTemperature="62"><Component bases="atatGCGGCCGCg"/><Component hybridizedRange="4579-4600" bases="cttggcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="14" name="KROL293 rpsU1_p2_R" sequence="tacttagcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4569-4600" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="ta"/><Component hybridizedRange="4598-4600" bases="ctt"/><Component bases="a"/><Component hybridizedRange="4569-4596" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4600" boundStrand="1" annealedBases="cttgcataccctctcacctcgcttaaatgcc" meltingTemperature="63"><Component bases="ta"/><Component hybridizedRange="4598-4600" bases="ctt"/><Component bases="a"/><Component hybridizedRange="4569-4596" bases="gcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="15" name="KROL295 rpsU2_p2_R" sequence="cgcttggcataccctctcacctcgcttaaatgcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4569-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component bases="cg"/><Component hybridizedRange="4569-4600" bases="cttggcataccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4600" boundStrand="1" annealedBases="cttggcataccctctcacctcgcttaaatgcc" meltingTemperature="67"><Component bases="cg"/><Component hybridizedRange="4569-4600" bases="cttggcataccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="16" name="KROL164 P035 INT_VSVG" sequence="TTTACCTAATCTATTCATTTCAATATCAG" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4804-4832" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="4804-4832" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite><BindingSite simplified="1" location="4804-4832" boundStrand="1" annealedBases="TTTACCTAATCTATTCATTTCAATATCAG" meltingTemperature="52"><Component hybridizedRange="4804-4832" bases="TTTACCTAATCTATTCATTTCAATATCAG"/></BindingSite></Primer><Primer recentID="17" name="KROL240 katg-ck-1" sequence="gattacgaattcttatttacctaatctat" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="4819-4836" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="4819-4836" bases="cttatttacctaatctat"/></BindingSite><BindingSite simplified="1" location="4819-4836" boundStrand="1" annealedBases="cttatttacctaatctat" meltingTemperature="40"><Component bases="gattacgaatt"/><Component hybridizedRange="4819-4836" bases="cttatttacctaatctat"/></BindingSite></Primer><Primer recentID="18" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="19" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="20" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="21" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc" dateAdded="2020-01-07T17:47:09Z"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="19:34:51">2020.1.22</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     24~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61DkAcYdU_Ej@TЛWQzDdtrUEVNUSi%UQMEc6ghVFHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i              [   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  H<?xml version="1.0"?><Features nextValidID="65"><Feature recentID="0" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="1" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="2" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="3" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment 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name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="18" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="19" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="20" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="21" name="3" type="misc_feature" 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name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="25" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="26" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="27" name="bla'" directionality="1" translationMW="22928.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="30" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="31" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="32" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="33" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="34" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="35" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="36" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="37" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="38" name="'ampR" directionality="1" translationMW="8646.90" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="48" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="49" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="50" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5212" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="51" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="52" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-4883" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4906-5003" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5100-5194" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature><Feature recentID="60" name="30S ribosomal protein S21" directionality="1" translationMW="7816.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4853" maxFusedRunOn="4853"><Segment range="4611-4808" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="61" name="rpsU3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4611-4808" color="#00e600" type="standard"/></Feature><Feature recentID="62" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4605-4808" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="63" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4818-4850" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4599-4853" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="1,2" nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="3" name="KROL42 OE_rpsU3R" sequence="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4779-4819" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4779-4819" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite><BindingSite simplified="1" location="4779-4819" boundStrand="1" annealedBases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt" meltingTemperature="67"><Component hybridizedRange="4779-4819" bases="ATAtGCGGCCGCataagaataagaccttcttgcttcttttt"/></BindingSite></Primer><Primer recentID="4" name="KROL41 OE_rpsU3F" sequence="atatGAATTCaggagggataatatgccaagaataatagttgacc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4631" boundStrand="0" annealedBases="GAATTCaggagggataatatgccaagaataatagttgacc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="4592-4631" bases="GAATTCaggagggataatatgccaagaataatagttgacc"/></BindingSite><BindingSite simplified="1" location="4592-4631" boundStrand="0" annealedBases="GAATTCaggagggataatatgccaagaataatagttgacc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="4592-4631" bases="GAATTCaggagggataatatgccaagaataatagttgacc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="21:42:47">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  \     n  X   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     .4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>_}ZQodڦYR&inJ*)ڙYEfDi/Xex61DkA	S)~bjUCd,zSנ75QUW8Q;eYJU6ghVFHNfSL0j/D]te\T?Kʦ9-@YxUV?{lW?52][с+-K9i              W   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="40" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" 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color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="53" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="54" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="55" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4851-4879" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="56" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4337" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="57" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4902-4999" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="58" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5096-5190" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="59" name="30S ribosomal protein S21" directionality="1" translationMW="7840.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4849" maxFusedRunOn="4849"><Segment range="4610-4804" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="60" name="rpsU2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4610-4804" color="#00e600" type="standard"/></Feature><Feature recentID="61" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4814-4846" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="62" name="rpsU2 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4592" color="#00ff00" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="5"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="4500-4528" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="4500-4528" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="4332-4367" boundStrand="0" annealedBases="ggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component bases="atat"/><Component hybridizedRange="4332-4367" bases="ggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="4569-4598" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="4569-4598" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="4569-4598" boundStrand="1" annealedBases="tgaattcccctctcacctcgcttaaatgcc" meltingTemperature="65"><Component bases="ata"/><Component hybridizedRange="4569-4598" bases="tgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer><Primer recentID="3" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4775-4815" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="4775-4815" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="4775-4815" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="4775-4815" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="4" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4592-4628" boundStrand="0" annealedBases="GAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4592-4628" bases="GAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="4592-4628" boundStrand="0" annealedBases="GAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4592-4628" bases="GAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  b<Notes>
<UUID>8ee8c658-0f46-44ae-a590-096168bc3473</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="17:4:46">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Hr        }   	      }fZmU8,j]񛆤     ߉RlDuUuUjUfz5^+cgVX]Ukf٭pˣ1k`c`&VbЌu`c=W`	2XbFGjc}6axWMBiYЅY3HkLlɫ.mŅ
1!q2X8>(]KQXn|{`ކ_@hbİ[4|gnoﭧEpU&[YZ4ja*[)[8mae\T?KʦK'9+;z8`k      >	!      ~     S4~M|Viݕ]#XRƪe:/jAmNq֣zVJ]T&:txךG*F樭*QhΜ6|jBÂ%C|l](>X3&ۈjf'[6JSw8s?.B|qP>g>jB-Y`rЃzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|X?WeQUVJx`JAVFx,UVVJy>:AkO6<2W]WǄcNDKiC*P7^>U?4:}eU|LV`Jinmt;NZ      |   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ?<?xml version="1.0"?><Features nextValidID="57"><Feature recentID="0" name="T0" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4621-4715" color="#ffffff" type="standard"/><Q name="gene"><V text="T0"/></Q><Q name="product"><V text="lambda T0 transcriptional terminator"/></Q></Feature><Feature recentID="1" name="T1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4427-4524" color="#a6acb3" type="standard"/><Q name="gene"><V text="T1"/></Q><Q name="product"><V text="E. coli rrnB T1 transcriptional terminator"/></Q></Feature><Feature recentID="2" name="mcs" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4321-4404" color="#a6acb3" type="standard"/><Q name="gene"><V text="mcs"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="3" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4288-4314" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="4" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4184-4213" color="#a020f0" type="standard"/></Feature><Feature recentID="5" name="Tn7L" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4312" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7L"/></Q><Q name="product"><V text="left end of Tn7"/></Q></Feature><Feature recentID="6" name="'mini-Tn7T" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4147-4733" color="#a6acb3" type="standard"/><Q name="gene"><V text="'mini-Tn7T"/></Q></Feature><Feature recentID="7" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3375-3375" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="8" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3374-3374" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="9" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3372-3372" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="10" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3371-3371" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="11" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3370" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="12" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3369-3369" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="13" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3368-3368" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="14" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3366-3366" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="15" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3365-3365" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="16" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3376" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="17" name="pUC ori" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3266-3933" color="#a6acb3" type="standard"/><Q name="gene"><V text="pUC ori"/></Q><Q name="product"><V text="pUC origin"/></Q></Feature><Feature recentID="18" name="'ampR" directionality="1" translationMW="8647.11" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2873-3115" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="'ampR"/></Q><Q name="product"><V text="ampicillin resistance (bla) ORF"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2787-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="20" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2786-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="21" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2784-2784" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="22" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2782-2782" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="23" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2780-2780" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="24" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2779-2779" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="25" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2778-2778" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="26" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2777-2777" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="27" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2776-2776" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="28" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2787" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="29" name="bla'" directionality="1" translationMW="22929.07" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3118" maxFusedRunOn="3118"><Segment range="2258-2872" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="bla'"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="30" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1982-2001" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="31" name="Tn7R" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="Tn7R"/></Q><Q name="product"><V text="right end of Tn7"/></Q></Feature><Feature recentID="32" name="mini-Tn7T''" directionality="2" type="misc_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1467-1676" color="#a6acb3" type="standard"/><Q name="gene"><V text="mini-Tn7T''"/></Q></Feature><Feature recentID="33" name="'MCS'" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#a6acb3" type="standard"/><Q name="gene"><V text="'MCS'"/></Q><Q name="product"><V text="multiple cloning site"/></Q></Feature><Feature recentID="34" name="'lacZ'" directionality="2" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1459-1462" color="#993366" type="standard"/><Q name="gene"><V text="'lacZ'"/></Q></Feature><Feature recentID="35" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1430-1454" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="36" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1391-1424" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="37" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1341-1368" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="38" name="rrnBT1" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1175-1218" color="#a6acb3" type="standard"/><Q name="gene"><V text="rrnBT1"/></Q><Q name="product"><V text="transcriptional terminator"/></Q></Feature><Feature recentID="39" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="40" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-1137" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="41" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-1136" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="42" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1134-1134" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="43" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1132-1132" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="44" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1130-1130" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="45" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1127-1127" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="46" name="Geneious type: mismatch" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1125-1125" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: mismatch"/></Q></Feature><Feature recentID="47" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="48" name="aphA-1" directionality="1" translationMW="30987.94" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="49" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="50" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="51" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="52" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="53" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="54" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="55" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="56" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>97da295a-ac06-4ba6-951b-43fb4ef45dc7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>Mini Tn7 plasmid.</Description>
<Created UTC="4:0:0">2016.10.14</Created>
<LastModified UTC="4:0:0">2016.10.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>SECID/File created by SciEd Central, Scientific &amp; Educational Software.</Comments>
</Notes>
        _          f	ٕ_Mn镝%)f$RyZy嘤YoyhVh$@v\WjS@         -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  I<?xml version="1.0"?><Features nextValidID="1"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-264" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  A<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL121 drpsU2 seq primer" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-09-30T18:31:13Z"><BindingSite location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="172-200" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="53"><Component hybridizedRange="172-200" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL290  rpsU2_promoter_F" sequence="atatggtaccaacctttaaccatataaatttctaaagaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="0-39" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component hybridizedRange="0-39" bases="atatggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatggtaccaacctttaaccatataaatttctaaagaat" meltingTemperature="58"><Component hybridizedRange="0-39" bases="atatggtaccaacctttaaccatataaatttctaaagaat"/></BindingSite></Primer><Primer recentID="2" name="KROL291  rpsU2_promoter_R" sequence="atatgaattcccctctcacctcgcttaaatgcc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2019-12-19T16:25:08Z"><BindingSite location="241-273" boundStrand="1" annealedBases="atatgaattcccctctcacctcgcttaaatgcc" meltingTemperature="64"><Component hybridizedRange="241-273" bases="atatgaattcccctctcacctcgcttaaatgcc"/></BindingSite><BindingSite simplified="1" location="241-273" boundStrand="1" annealedBases="atatgaattcccctctcacctcgcttaaatgcc" meltingTemperature="64"><Component hybridizedRange="241-273" bases="atatgaattcccctctcacctcgcttaaatgcc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>60404d3f-5659-4a91-8a87-2be68693a367</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:25:13">2019.12.19</Created>
<LastModified UTC="16:25:13">2019.12.19</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       u  *        *Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf@2iQQ,FQ1T8I^rgU=zsUUxUQ5dP0fxfadi	YAVfz|}+	U]=TU^wNDhsUhXzdl쑤Nix(Wz}YQSUFTӕ]EG)5;9MY.k45GU	)^Sh7GONtUl:.PU[iAIk[YSPƮ>CS5i1jTR>Jc[TMlNNP Q[]ETD$ЕZ1Te55vǳTM+`Gc&;QitV]M9w5S]GSaJB$EK^zCFUMfNdQTT$l{jem9)[mZn*V滪[+cWϙ:wiZIRNKEb}Zz;jD%d!b^f)\yncQz^fuTOI%V旮UY[ՂNk૪媩eWjhZԛmfؕYvYѹޗuKF:Ƃb[ITꎕ9yxf[bamUf񖆖#Y骕jn(^	[UٶgYmyM㢳RReYꕅY%%I(b X}a^ZT:_JNnnNm~!^n_hC^Qf+9uiMکz-fukWi%]yeURJuTZRRJkUHgUI*U#S5iUUufeDY@UoEy''`jFתEaykX]UmyUQM(rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]V9PSܑSEpabӞ\Q4$`NG©Sh(?RWPn3@QmDtAh]F
:CØxG[;<+ËL+Q#X^4CLÛ 4;P91Hoߣ<EI0Ío~?k}LWlr{<.AqD@;3C3cn!f UΫ A2fNX:#l3lϮhUADIv"jTF8Q#q )]0o?*aީR &hsM{<kXz
{}-TۧIDZcH}]^uu m{>|=WL}>$u3W^^z{]idx(}?
8en	7=%?&)p5ѳd#6^-RfzWPnBo[UJxLF2[:5uU}L mD׺	x:Dg$JCz^Mg9o} H1.G)e7t:|^2EL:PPЛSt3q: W>	/;- W=5>p;v+) ei|O4Ĵ     F  H    (  (   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="14"><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7466" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4716-4765" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="2" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="810-842" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="3" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="971-993" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL3"/></Q></Feature><Feature recentID="4" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q><Q name="note"><V text="AROL2"/></Q></Feature><Feature recentID="5" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5666-6523" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="6" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4854-5645" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="7" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2659-3675" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="8" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2653" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="9" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1136-2011" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="10" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="11" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-800" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="12" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-800" color="#00e600" type="standard"/></Feature><Feature recentID="13" name="30S ribosomal protein S21" directionality="1" translationMW="7840.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="845" maxFusedRunOn="845"><Segment range="606-800" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL40 OE_rpsU2R" sequence="atatGCGGCCGCccttttaactattatattgctcttatgag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite><BindingSite simplified="1" location="771-811" boundStrand="1" annealedBases="atatGCGGCCGCccttttaactattatattgctcttatgag" meltingTemperature="65"><Component hybridizedRange="771-811" bases="atatGCGGCCGCccttttaactattatattgctcttatgag"/></BindingSite></Primer><Primer recentID="1" name="KROL39 OErpsU2F" sequence="atatGAATTCaggaggagggtatgccaagcgttagaattaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite><BindingSite simplified="1" location="584-624" boundStrand="0" annealedBases="atatGAATTCaggaggagggtatgccaagcgttagaattaa" meltingTemperature="65"><Component hybridizedRange="584-624" bases="atatGAATTCaggaggagggtatgccaagcgttagaattaa"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="16:32:46">2019.1.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       u  +        +Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пfy9IIMJW\UN	5FCM%O]TTdsDZDQ6<ٞXYBVPoy;*է|^JBbj26oUWmOU:W]ӵ9%_㨥4\Z-:$i$^
ޟVTiTUaQ4WDjQ橴ueFsSVEKyQUB@5
tW%95U[*N 1`5EZDhERdZTElj1MgZtZ$E*Oe[ST}VjWDEU%Q	4%CLU.MM`]q@iE:ɎdfCZ]!"UnSN]MTQXy	F%מѕSYU9vI[njJVVۢEVNiZVjRTSXVAaN뚫/d.Y)tXk-i[^n~u9Iuz릦*jVjkkzV`*jfYU/־&uY%a.VhntnB䭷zRюnU:k~^%zتod)YeHAzjZ8BVmms[^fgj:n:zzaVIxIR{J&؈6XWV7fcWғx:Gi#ih[:_FC韈WהY*%g]DGd殣jv^EKiZUIW^zUTqU$VTuTZRRJjUHTbMgDUUnfeDYQnU[GGo^gII5|X^nDU[da^D+UTSJ),Aܬ-Nl @:7W` =dp(+dx]je<j悕VZ1jU5Tw$T\#XuW53TEM	:410`tZ=8?%
OT`nQl6QsB΀uÎ<4?
|~/08=+8(0hR<ARom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮZPQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10     F  H     )  )  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="601-801" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="801-801" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="783" maxFusedRunOn="783"><Segment range="801-801" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="607-801" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="846" maxFusedRunOn="846"><Segment range="607-801" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-607" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="601-607" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1137-2012" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2235-2654" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2660-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4855-5646" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5667-6524" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="972-994" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="811-843" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4717-4766" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7467" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:52:23">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       t  %        %Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfcD_jEzTSQ:Dd4rTUEFMW=N)TMEEjh͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
POzYMޑh?Pi9\eUB
CVT^lUF]&LemeOVƩC^OuLŨDQRJ)mQ6U:U;u@GEo֨XUtDZ)RUBT;hDRڷ-Q4XFFd52/X iv4߄MuaNEHQ)`b]-yU59EQSd@jmjm(y[[l_>d몸Veke%KU:-.jK앒@>ꛪyriպMIEz^YS=$_)[^jjbVffwoV	:a낮j5^nkRmWbVbfgF.Jz]׮.	o&S:V닸Wn}!-BUZ[	dUYz%mV[fg5i6ϖIIgVd'$mia{5iSx.:f5})9ʊkOgv6{c:Qd:n{}yERPz5IvNj=6jd^Dծe^u筕UI*RJkUHgUI*U!U$VTO$ztAUVfTEf jUt{TzVzcWZk^`dIuUK%BUE4bD=V
#~Uz@
ܒ?G
?r/rHWuꆦPƬ>h)Ua8j]UsuZ3P8ALrEOK8NxnPsS5DTГH9
ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
d3L, 
xF~tNa3@ECBo	L4D\&d,
߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H    #  #  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  _<?xml version="1.0"?><Features recycledIDs="9" nextValidID="19"><Feature recentID="8" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="595-795" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="7" name="FTL_0455" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="795-795" color="#00e600" type="standard"/></Feature><Feature recentID="6" name="acetyltransferase" directionality="2" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="173" maxRunOn="777" maxFusedRunOn="777"><Segment range="795-795" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="601-795" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7847.44" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="840" maxFusedRunOn="840"><Segment range="601-795" color="#ff0000" type="standard" translated="1"/><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="FTL_0457" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="595-601" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="cold shock protein" directionality="1" translationMW="131.18" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="67" hitsStopCodon="1"><Segment range="595-601" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1131-2006" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2229-2648" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2654-3670" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4849-5640" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5661-6518" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="966-988" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="805-837" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4711-4760" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7461" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:57">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       A  \        \Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&Пf6ghVFNʵil+קҰИUSNUttNFW)M5VKfαI>旂wՕU1XTeM9UTym"sYQՑR渣C^T~P*MB<U	e6czD}DAMqVʓ)EXQyeVt1?Q[jxd5?Y֝3:EJ*5DVTqZaUhUITBM	PKSSXj{8DQbv2c֗@HU`۔ӗ~U5y9i!D-DQt4eTdEENjFǺfVۚթ袡nQnkU5|Zj.}ک.T贻V'ժ~jgSKY.~KVJn n%byoV6=%e{g]eNd]~*niz驪Z޾սX$醻
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UҊa)k+<:S[ ;+ƤUi+rHl*Y {^{_.k@jUuVZk@@0]=/4W-99@ULSBN :vdx*51|N 	BS%x:<4GA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+TIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLAL     F  H  Q  Z  Z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A<?xml version="1.0"?><Features recycledIDs="2,3,4,5,6,7,8,9" nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="930-1805" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2447" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2453-3469" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4648-5439" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-6317" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="765-787" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="604-636" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4510-4559" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7260" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:51:53">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  Lg5|XӃqЫT5ib:cɊ"]jWNas'KSjgY^fbJUUUVNzhQZcuVZTQ]Q&ПfP)iYn!CNUD
[!3Wd[a,Fh&=eWREfYTYg9QiPiyOjD䓑iS,n	TXQZFF*;pkUQ)3NfuX$TwNVf]E'f6ZTUjTRZP͙%eZ|a4&&#kUvSUzu;]U:SEUaٳFO9]eFLeVSNUtFjH\T`55dT(וT$
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ML_Ӏ3P5I^=A` mFaʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J녡Ua&ڼ?>*W8!RUFҀĀl^tQJ\zJ㘃0oɡQ5,gya(IESn6%i#ew=zzΪ`%(N}\q2X5(^ix1yNu`CTA388*hᕸ .&4 `$qט\VGF	x}JٙF^ZAQn}V!*2$UTon8U}0^%M4g՞+H)}Q{7ϝ)= RȺ|
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߸z,j 0O\0¾8>خɧϨHN<\|LS      F  H        !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="299-533" color="#ffffff" type="standard"/><Q name="note"><V text="GroE"/></Q></Feature><Feature recentID="17" name="ORF3" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1635-2510" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF3"/></Q></Feature><Feature recentID="16" name="ORF2" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2733-3152" color="#a6acb3" type="standard"/><Q name="note"><V text="ORF2"/></Q></Feature><Feature recentID="15" name="RepA" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-4174" color="#a6acb3" type="standard"/><Q name="note"><V text="RepA"/></Q></Feature><Feature recentID="14" name="kanamycin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5353-6144" color="#a6acb3" type="standard"/><Q name="note"><V text="kanamycin"/></Q></Feature><Feature recentID="13" name="ampicillin" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6165-7022" color="#a6acb3" type="standard"/><Q name="note"><V text="ampicillin"/></Q></Feature><Feature recentID="12" name="PCR_primer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="436-458" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL2"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="11" name="PCR_primer" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1470-1492" color="#a6acb3" type="standard"/><Q name="note"><V text="AROL3"/></Q><Q name="label"><V text="nonstandard type:  PCR_primer"/></Q></Feature><Feature recentID="10" name="VSV-G" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1309-1341" color="#993366" type="standard"/><Q name="note"><V text="VSV-G"/></Q></Feature><Feature recentID="9" name="P018 FTL_0552fragF" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="773-799" color="#a020f0" type="standard"/><Q name="note"><V text="P018 FTL_0552fragF"/></Q></Feature><Feature recentID="8" name="P044 INTpEX_FTL0552" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="686-713" color="#a020f0" type="standard"/><Q name="note"><V text="P044 INTpEX_FTL0552"/></Q></Feature><Feature recentID="7" name="P238 FTL_0552F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="616-642" color="#a020f0" type="standard"/><Q name="note"><V text="P238 FTL_0552F"/></Q></Feature><Feature recentID="6" name="P301 PmrA_comp_F" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-627" color="#a020f0" type="standard"/><Q name="note"><V text="P301 PmrA_comp_F"/></Q></Feature><Feature recentID="5" name="P017 FTL_0552fragR" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1272-1299" color="#a020f0" type="standard"/><Q name="note"><V text="P017 FTL_0552fragR"/></Q></Feature><Feature recentID="4" name="FTL_0552" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="616-1299" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="db_xref"><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="3" name="FTL_0552" directionality="1" translationMW="25520.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1344" maxFusedRunOn="1344"><Segment range="616-1299" color="#993366" type="standard" translated="1"/><Q name="inference"><V bool="1" text="18" predef="profile"/></Q><Q name="product"><V text="two-component response regulator"/></Q><Q name="note"><V text="/dnas_title=two-component response regulator"/></Q><Q name="locus_tag"><V text="FTL_0552"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513318.1"/></Q><Q name="db_xref"><V text="89255956" predef="GI"/><V text="3952730" predef="GeneID"/></Q></Feature><Feature recentID="2" name="P229 PmrADel5R" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="600-624" color="#a020f0" type="standard"/><Q name="note"><V text="P229 PmrADel5R"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5215-5264" color="#ffffff" type="standard"/><Q name="note"><V text="pCR2.1 NEOKAN promoter"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7965" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pF-PmrA-VSVG"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>d4656186-27b4-4203-b8dd-491ab5a1565e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:48:21">2018.12.19</Created>
<LastModified UTC="0:48:21">2018.12.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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recentID="67" name="Geneious type: primer_bind_reverse" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1103-1138" color="#a6acb3" type="standard"/><Q name="note"><V text="Geneious type: primer_bind_reverse"/></Q></Feature><Feature recentID="68" name="aphA-1" directionality="1" translationMW="30987.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="309-1124" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="aphA-1"/></Q><Q name="product"><V text="aminoglycoside acetyltransferase"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="69" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="219-219" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="transcriptional start site"/></Q></Feature><Feature recentID="70" name="primer binding site" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-230" color="#a020f0" type="standard"/></Feature><Feature recentID="71" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="208-208" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="promoter -10"/></Q></Feature><Feature recentID="72" name="(null)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-184" color="#a6acb3" type="standard"/><Q name="gene"><V text="(null)"/></Q><Q name="product"><V text="-35 promoter"/></Q></Feature><Feature recentID="73" name="PgroESL" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="184-219" color="#993366" type="standard"/><Q name="gene"><V text="PgroESL"/></Q><Q name="product"><V text="F. tularensis groESL promoter"/></Q></Feature><Feature recentID="74" name="3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="98-122" color="#a6acb3" type="standard"/><Q name="gene"><V text="3"/></Q><Q name="product"><V text="resolvase binding site III"/></Q></Feature><Feature recentID="75" name="2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="59-92" color="#a6acb3" type="standard"/><Q name="gene"><V text="2"/></Q><Q name="product"><V text="resolvase binding site II"/></Q></Feature><Feature recentID="76" name="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9-35" color="#a6acb3" type="standard"/><Q name="gene"><V text="1"/></Q><Q name="product"><V text="resolvase binding site I"/></Q></Feature><Feature recentID="77" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4608-4616" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="78" name="FTL_0126" directionality="1" type="gene" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4608-4616" color="#00e600" type="standard"/></Feature><Feature recentID="79" name="hypothetical protein" directionality="1" translationMW="360.41" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="4" maxRunOn="7694" maxFusedRunOn="7694"><Segment range="4608-4616" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VKD"/></Q></Feature><Feature recentID="80" name="pdpA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4608-4616" color="#a6acb3" type="standard"/></Feature><Feature recentID="81" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="82" name="FTL_0420" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4444" color="#00e600" type="standard"/></Feature><Feature recentID="83" name="hypothetical protein" directionality="1" translationMW="3690.28" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="90" hitsStopCodon="1"><Segment range="4339-4444" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="AKAGKSVDFMASDTACKTYNLLVNENRNVSCIII*"/></Q></Feature><Feature recentID="84" name="tul4 promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4339-4488" color="#a6acb3" type="standard"/></Feature><Feature recentID="85" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4623-4625" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEX"/></Q></Feature><Feature recentID="86" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4623-4625" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="87" name="lacZ" directionality="1" translationMW="89.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7694" maxFusedRunOn="7694"><Segment range="4623-4625" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacZ&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-galactosidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="A"/></Q></Feature><Feature recentID="88" name="source" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4623-4625" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pEXPFTL_0702-lacZ"/></Q></Feature><Feature recentID="89" name="potential TSS" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4489" color="#a6acb3" type="standard"/></Feature><Feature recentID="90" name="PRE" directionality="1" type="misc_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4527-4533" color="#0080ff" type="standard"/><Q name="note"><V text="PRE"/></Q></Feature><Feature recentID="91" name="-35" directionality="1" type="-35_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4540-4545" color="#00ff00" type="standard"/><Q name="note"><V text="-35"/></Q></Feature><Feature recentID="92" name="-10" directionality="1" type="-10_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4563-4568" color="#00ff00" type="standard"/><Q name="note"><V text="-10"/></Q></Feature><Feature recentID="93" name="Francisella tularensis subsp. holarctica" type="source" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4574" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="94" name="5'UTR pdpA extended" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4574" color="#a6acb3" type="standard"/></Feature></Features>
  4<?xml version="1.0"?><Primers nextValidID="22"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL134 GroEL rbs FR2 R2" sequence="atatGGTACCcaagagcttggaactttgagattgtt"><BindingSite location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite><BindingSite simplified="1" location="219-244" boundStrand="0" annealedBases="caagagcttggaactttgagattgtt" meltingTemperature="58"><Component bases="atatGGTACC"/><Component hybridizedRange="219-244" bases="caagagcttggaactttgagattgtt"/></BindingSite></Primer><Primer recentID="1" name="KROL90 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="2" name="KROL154 PCR primer mod groel rbs r" sequence="ctttgagattgttctaagatgcataca"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="3" name="KROL43 Seq_pF_1F" sequence="atctagacttgcaagagcttggaac"><BindingSite location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite><BindingSite simplified="1" location="208-232" boundStrand="0" annealedBases="atctagacttgcaagagcttggaac" meltingTemperature="59"><Component hybridizedRange="208-232" bases="atctagacttgcaagagcttggaac"/></BindingSite></Primer><Primer recentID="4" name="KROL257 pEX_Seq_VSVG" sequence="ctgataccgctcgccgcagcc"><BindingSite location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite><BindingSite simplified="1" location="3995-4015" boundStrand="0" annealedBases="ctgataccgctcgccgcagcc" meltingTemperature="66"><Component hybridizedRange="3995-4015" bases="ctgataccgctcgccgcagcc"/></BindingSite></Primer><Primer recentID="5" name="KROL154 PCR primer mod groel rbs ra" sequence="ctttgagattgttctaagatgcataca" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite><BindingSite simplified="1" location="232-258" boundStrand="0" annealedBases="ctttgagattgttctaagatgcataca" meltingTemperature="55"><Component hybridizedRange="232-258" bases="ctttgagattgttctaagatgcataca"/></BindingSite></Primer><Primer recentID="6" name="P570 Tn_Mar1" sequence="tttgtcaaacaagctctcgattttaac" description="0"><BindingSite location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite><BindingSite simplified="1" location="140-166" boundStrand="1" annealedBases="tttgtcaaacaagctctcgattttaac" meltingTemperature="57"><Component hybridizedRange="140-166" bases="tttgtcaaacaagctctcgattttaac"/></BindingSite></Primer><Primer recentID="7" name="P049 groESpromoF" sequence="atatCTGCAGgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter F; F. tularensis LVS; Discarded! Wrong enzyme site"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatCTGCAGgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="8" name="P051 groESpromoR" sequence="atatGGATCCgaatgttcataacaatcttactcctttg" description="To amplify groES (FTL_1715) promoter for outward-facing promoter R BamHI; F. tularensis LVS"><BindingSite location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite><BindingSite simplified="1" location="290-304" boundStrand="1" annealedBases="aatcttactcctttg" meltingTemperature="41"><Component bases="atatGGATCCgaatgttcataac"/><Component hybridizedRange="290-304" bases="aatcttactcctttg"/></BindingSite></Primer><Primer recentID="9" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4656-4675" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4656-4675" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="4656-4675" boundStrand="1" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="4656-4675" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="10" name="KROL170 pKR34_lacZ_R" sequence="atatCaattgTTATTTTTGACACCAGACCAACTGGT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7668-7693" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7668-7693" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite><BindingSite simplified="1" location="7668-7693" boundStrand="1" annealedBases="TTATTTTTGACACCAGACCAACTGGT" meltingTemperature="57"><Component bases="atatCaattg"/><Component hybridizedRange="7668-7693" bases="TTATTTTTGACACCAGACCAACTGGT"/></BindingSite></Primer><Primer recentID="11" name="KROL177 pKR34_seq_3" sequence="GAGGCCGATACTGTCGTCGTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="4859-4880" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4859-4880" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite><BindingSite simplified="1" location="4859-4880" boundStrand="0" annealedBases="GAGGCCGATACTGTCGTCGTCC" meltingTemperature="64"><Component hybridizedRange="4859-4880" bases="GAGGCCGATACTGTCGTCGTCC"/></BindingSite></Primer><Primer recentID="12" name="KROL178 pKR34_seq_4" sequence="CTACGGGTAACAGTTTCTTTATGGC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5381-5405" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5381-5405" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite><BindingSite simplified="1" location="5381-5405" boundStrand="0" annealedBases="CTACGGGTAACAGTTTCTTTATGGC" meltingTemperature="57"><Component hybridizedRange="5381-5405" bases="CTACGGGTAACAGTTTCTTTATGGC"/></BindingSite></Primer><Primer recentID="13" name="KROL179 pKR34_seq_5" sequence="ATCCGCGCTGGCTACCGGCGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5907-5927" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5907-5927" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite><BindingSite simplified="1" location="5907-5927" boundStrand="0" annealedBases="ATCCGCGCTGGCTACCGGCGA" meltingTemperature="70"><Component hybridizedRange="5907-5927" bases="ATCCGCGCTGGCTACCGGCGA"/></BindingSite></Primer><Primer recentID="14" name="KROL180 pKR34_seq_6" sequence="TGAACGGTCTGGTCTTTGCCGAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6429-6451" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6429-6451" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite><BindingSite simplified="1" location="6429-6451" boundStrand="0" annealedBases="TGAACGGTCTGGTCTTTGCCGAC" meltingTemperature="64"><Component hybridizedRange="6429-6451" bases="TGAACGGTCTGGTCTTTGCCGAC"/></BindingSite></Primer><Primer recentID="15" name="KROL181 pKR34_seq_7" sequence="ACGCCGCTGCGCGATCAGTTCA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6953-6974" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6953-6974" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite><BindingSite simplified="1" location="6953-6974" boundStrand="0" annealedBases="ACGCCGCTGCGCGATCAGTTCA" meltingTemperature="66"><Component hybridizedRange="6953-6974" bases="ACGCCGCTGCGCGATCAGTTCA"/></BindingSite></Primer><Primer recentID="16" name="KROL182 pKR34_seq_8" sequence="CTTCCAGTTCAACATCAGCCGCTA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7483-7506" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7483-7506" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite><BindingSite simplified="1" location="7483-7506" boundStrand="0" annealedBases="CTTCCAGTTCAACATCAGCCGCTA" meltingTemperature="59"><Component hybridizedRange="7483-7506" bases="CTTCCAGTTCAACATCAGCCGCTA"/></BindingSite></Primer><Primer recentID="17" name="KROL319  LacZ_construct_r" sequence="atatggatcCTTATTTTTGACACCAGACCAACTGG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7669-7700" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7669-7700" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite><BindingSite simplified="1" location="7669-7700" boundStrand="1" annealedBases="tggatcCTTATTTTTGACACCAGACCAACTGG" meltingTemperature="64"><Component bases="ata"/><Component hybridizedRange="7669-7700" bases="tggatcCTTATTTTTGACACCAGACCAACTGG"/></BindingSite></Primer><Primer recentID="18" name="KROL326  tul4_promoter_construct_r" sequence="atatggtacccgcaaaagctggtaaaagtgttgatt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:30:36Z"><BindingSite location="4332-4363" boundStrand="0" annealedBases="ggtacccgcaaaagctggtaaaagtgttgatt" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="4332-4363" bases="ggtacccgcaaaagctggtaaaagtgttgatt"/></BindingSite><BindingSite simplified="1" location="4332-4363" boundStrand="0" annealedBases="ggtacccgcaaaagctggtaaaagtgttgatt" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="4332-4363" bases="ggtacccgcaaaagctggtaaaagtgttgatt"/></BindingSite></Primer><Primer recentID="19" name="KROL331  pKR71_reverse_frag1" sequence="gtgtattgataaatcgattataacattaattaaaataaaataa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:32:04Z"><BindingSite location="4455-4497" boundStrand="1" annealedBases="gtgtattgataaatcgattataacattaattaaaataaaataa" meltingTemperature="54"><Component hybridizedRange="4455-4497" bases="gtgtattgataaatcgattataacattaattaaaataaaataa"/></BindingSite><BindingSite simplified="1" location="4455-4497" boundStrand="1" annealedBases="gtgtattgataaatcgattataacattaattaaaataaaataa" meltingTemperature="54"><Component hybridizedRange="4455-4497" bases="gtgtattgataaatcgattataacattaattaaaataaaataa"/></BindingSite></Primer><Primer recentID="20" name="KROL332  pKR71_forward_frag2" sequence="taatcgatttatcaatacactttttataactttatcgtc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-09-02T15:34:35Z"><BindingSite location="4478-4516" boundStrand="0" annealedBases="taatcgatttatcaatacactttttataactttatcgtc" meltingTemperature="57"><Component hybridizedRange="4478-4516" bases="taatcgatttatcaatacactttttataactttatcgtc"/></BindingSite><BindingSite simplified="1" location="4478-4516" boundStrand="0" annealedBases="taatcgatttatcaatacactttttataactttatcgtc" meltingTemperature="57"><Component hybridizedRange="4478-4516" bases="taatcgatttatcaatacactttttataactttatcgtc"/></BindingSite></Primer><Primer recentID="21" name="KROL349  pdpA_6aa_NotI" sequence="atatGCGGCCGCatcttttactgctatcattgtctactta" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2020-10-08T15:59:15Z"><BindingSite location="4588-4623" boundStrand="1" annealedBases="GCGGCCGCatcttttactgctatcattgtctactta" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4588-4623" bases="GCGGCCGCatcttttactgctatcattgtctactta"/></BindingSite><BindingSite simplified="1" location="4588-4623" boundStrand="1" annealedBases="GCGGCCGCatcttttactgctatcattgtctactta" meltingTemperature="65"><Component bases="atat"/><Component hybridizedRange="4588-4623" bases="GCGGCCGCatcttttactgctatcattgtctactta"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>d613bae7-cfe9-4093-aaf7-ae963f338557</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:58:27">2019.12.19</Created>
<LastModified UTC="16:4:1">2020.10.8</LastModified>
<CreatedBy>Hannah</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         6+ Cutters                                                                                                   d      HO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
