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u. 
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^

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Sy..z 7dt'yPk<-    !8FiN	gq5[zFe{#4I"j[ l `n	DNc}Pl7t:#   1kK6i	


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 m_q!*EI A%	Mm;	##vN=~%6 v3#;U	I	Q		r]6\DO4;WZfq'o& 6|4Y
_B-_k5 {;I}x
9YiQM&QaCer)/ JU\c&,A
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tn`	+>  x Pz'u
#N

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_\ hO
a BRq(			f$& R <	

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R

5k7w(Fl>G kR		M tPtp,{4 ?w4$rGVcJ		v
	s*!](7<NTX[dG+]mX0 V<PE	4	:`^G,2j}5r( b0	mz77E.g~<^! 
ICZ	 	c


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ntU`<bi|2mISA0.X .X}6df I!l#  # y
[]b[Nt>.L*0/ p<H~>			Y
 d:FZjR''>Dfs'  ,z^$&8RV<`
X#  $|!NT{JGzj. b%c- | 5]0'-		=}  KEB	#J! Udz	
-|"}> ?QVtlCsV24 = `  
'u	
>o$
G0P!  dy.<^03N	

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:JnYBU  {vI[9G^y QZnSW#  K ]o$sd9a   Er f	s
3qsi`   ,u			.}  d'gjK C	`3M GeGZjpp% 8 o     ci-?&EA ,24C]" + r ;*OS	8
H
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x^iDbV =.Cx				
1%h G-T%;E6Xl" S =	
e
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Ici#
w$  w4S


-l|)(C5HFp~%%  Heo\	p
6X058xqUDM7$ \  'jk
~lpCHPnmJ 1 ,  (:{pq	

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M-|&Oz3H@ x+z%sWK		Q
!ot+DL^)8DUo]qhY6 @~5LH|w

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s=\3 Q N8=QnQ
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		5	?		
;alHR@R3)/T~SJ%   "  oSF			%U_-~Wb, { 4,{k<B|U= _  x&/t1	=XqF/R Ymg
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@2+1L;  i  )p0?ZDg	8	


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r
n@{
y2p#* ! nM7R						
$.r5?5hV}
[a+  l%W)jATwF    (ao+>yf7	




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q	a#Gm?-qT>g	1o|	Zq     /`P=x23vw 	




opKLMuF
-=>cdjklm*+,_nopU={/Y	3 04ax	d			

,1 BNe[`xF#+8
h  		2,9M m|, }/h Y!5b	

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)7F\eyJ"% *~@Y	e	-IgKQV\i  	A
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eg:g |8+ f="c<&		"D[k`Sg (b%:E)ty S~p		!Gd(   ;c	


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'8DkG] )KT 
:$		9;%fYY  AUZ	/W.  +D}O){k%J$ %\K
.'R&] "|Hf		hLRcdjw 6Y^
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7  .1-		

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.2N +~7| WJ	 .A+$?PoH, V	

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m[!;Dt\*$   @4f1EyOQYamr5"& % #t+wL(QcDuWv'- > 1}	$

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LU^bx0pz@#OQb$ G a a (
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
tbvu  ;hT+  z	8hqB}  T["1@
,W@0 gFg'			!^u! >Uqb3	u
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Z#E\aZ&-;=s&  T	"
 `			vo#kF) ( 5  tHme  Jq+t	r
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iJ]o3o#  <-^Mw		i
O*01bg' W


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kBGO'pTLo$  
Z v ${~	CKBC\g `m	P
TQ&LZV ^ z  3w.s+;bUo{%  h  h	

69\ 3H   |)K

*6 '.nw1	


0J"El,)^*7f_z!  |)g? 
(^EHJL :U[ Ulm	vYZg_tz& BRo;%	f<T9(c7   =9		:gXJ$ZS C[	&Qv   %?-U^	
FP/ $Bi	p *e	bM V 
y*j'  "L*sJ[	%P0t :Xl!%  P|} z&K@T]rTw%Yxa  !gtjA/ ,E~LTF}	t
l4e,~r ' 4  s*s	j% ]VLv	N/SNIn u v-lr	~BTf!  &f	

/!]+(6e) T<	P8XI'^y  >#~ITh		
i)kL S\$i e	O7MSdemx }g	(hkl  0<  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                       1                                                            
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
   
                                                                                                                                                                                                                                                               ,               
                                                                      "                                          
                              
   
                     	                              '               	                                                	                                                            '   (      (   #         $            w      %   b   a   &         6   e                                                                                                                                                                            1                            
                                                                                                                                                                                                                                                                  
 
                                                                                                     ,     
                          "                
          
 
       	          '     	                	                    ' (  ( #   $    w  % b a &   6 e               
3u I	*r)*/"/1 #q
^OR4cccc:EXvS 'OR[dS<;E)GB
}
}
} 5I&Zc e DkDNR	 y	[(y	:( N^r^*	
wQ,{3^j :ry	C|o
oCdo
Cl"o
Cdb.	zr.	$^ sTL$ruCwOArW	^ 67<d,=}|S\m.$]EOs6<v		o
oCdof&		:=JS4 $QF
yKmqu2EOSDX\s
	,="xh6#t	modD| :.  
I kOjdn
k(o

F:C g  f"\cK&			!eU 	 A$EO .
<:Zl!lLZ l67	'K[ue
o1dixb6 #zi$i hp YZ5		8	
XK|&T]wIan	#		$	d- 0  	9F&oSn#<'R	?Zo"Fq >~T	
		:~p'q- 0  	9F&oSn#LF,~r]	| -} x )- 0  !p)r	T
vOX9F&oSn# >~T|	
&T]wIanxW
Y<%&c2i

4j'E) 6 ($h } %x

i M y-B<Ti
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    \  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="4,5,6" nextValidID="20"><Feature recentID="3" name="30S ribosomal protein S21" directionality="2" translationMW="866.05" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6659-6685" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="2" name="rpsU" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6659-6685" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6659-7261" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7990" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7262-7266" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7696-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7288-7304" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  B<?xml version="1.0"?><Primers recycledIDs="6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="5" name="KROL127 ∆rpsU3 FR2 R" sequence="ttttaatgaaaattaggtattttagttaaatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7229-7260" boundStrand="1" annealedBases="ttttaatgaaaattaggtattttagttaaatc" meltingTemperature="50"><Component hybridizedRange="7229-7260" bases="ttttaatgaaaattaggtattttagttaaatc"/></BindingSite><BindingSite simplified="1" location="7229-7260" boundStrand="1" annealedBases="ttttaatgaaaattaggtattttagttaaatc" meltingTemperature="50"><Component hybridizedRange="7229-7260" bases="ttttaatgaaaattaggtattttagttaaatc"/></BindingSite></Primer><Primer recentID="0" name="KROL126 ∆rpsU3 FR1 F" sequence="atctacctgattatttacttattttgataa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6136" boundStrand="0" annealedBases="atctacctgattatttacttattttgataa" meltingTemperature="51"><Component hybridizedRange="6107-6136" bases="atctacctgattatttacttattttgataa"/></BindingSite><BindingSite simplified="1" location="6107-6136" boundStrand="0" annealedBases="atctacctgattatttacttattttgataa" meltingTemperature="51"><Component hybridizedRange="6107-6136" bases="atctacctgattatttacttattttgataa"/></BindingSite></Primer><Primer recentID="1" name="KROL125 ∆rpsU3 FR2 F" sequence="atatGCGGCCGCtcttggcatattatcctctcttaatttt" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6668-6703" boundStrand="0" annealedBases="GCGGCCGCtcttggcatattatcctctcttaatttt" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="6668-6703" bases="GCGGCCGCtcttggcatattatcctctcttaatttt"/></BindingSite><BindingSite simplified="1" location="6668-6703" boundStrand="0" annealedBases="GCGGCCGCtcttggcatattatcctctcttaatttt" meltingTemperature="66"><Component bases="atat"/><Component hybridizedRange="6668-6703" bases="GCGGCCGCtcttggcatattatcctctcttaatttt"/></BindingSite></Primer><Primer recentID="2" name="KROL124 ∆rpsU3 FR1 R" sequence="atatGCGGCCGCTtcttattagttcaagtcataaagctatc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6639-6675" boundStrand="1" annealedBases="GCGGCCGCTtcttattagttcaagtcataaagctatc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6639-6675" bases="GCGGCCGCTtcttattagttcaagtcataaagctatc"/></BindingSite><BindingSite simplified="1" location="6639-6675" boundStrand="1" annealedBases="GCGGCCGCTtcttattagttcaagtcataaagctatc" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6639-6675" bases="GCGGCCGCTtcttattagttcaagtcataaagctatc"/></BindingSite></Primer><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7315-7334" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7315-7334" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7315-7334" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7315-7334" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:43:57">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAiY-DVf|Yi-a]Uөffedٵ)Y9fXXUo!ŽYVzHVik蚪b*InnubE&tz*IR8*ިeQiTUӞ^Rda^YD6iiE+PVidTjD:{Y%RoQr]}FI(njYfXhoUIUYV)f_ZUUUe&NEa&iYZ`M*mjY_fXe$TjFdefQgU	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    
    [  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="4,5,6" nextValidID="20"><Feature recentID="3" name="30S ribosomal protein S21" directionality="2" translationMW="842.11" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6598" maxFusedRunOn="6598"><Segment range="6659-6684" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="2" name="rpsU" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6659-6684" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6659-7260" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7989" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7261-7265" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7695-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7287-7303" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,1,0,5,6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7314-7333" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7314-7333" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7314-7333" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7314-7333" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:18:33">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *       -        -.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAiY-DVf|Yi-a]Uөffedٵ)Y9fXXUo!ŽYVzHVik蚪b*InnubE&tz*IR8*ިeQiTUӞ^Rda^YF6iiE+PVidTjD:{Y%RoQr]}FI(njYfXhoUIUYV)f_ZUUUe&NEa&iYZ`M*mjY_fXe$TjFdefQgU	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%    S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="4,5,6" nextValidID="20"><Feature recentID="3" name="30S ribosomal protein S21" directionality="2" translationMW="652.78" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6659-6676" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="2" name="rpsU" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6659-6676" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6659-7252" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7981" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7253-7257" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7687-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7279-7295" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,1,0,5,6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7306-7325" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7306-7325" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7306-7325" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7306-7325" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:16:18">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *       0        0.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAiY-DVf|Yi-a]Uөffedٵ)Y9fXXUo!ŽYVzHVik蚪b*InnubE&tz*IR8*ިeQiTUӞ^Rda^YE٦ZYDBYUYRnTQjyduIFvivfecj&XeaU'khjUeXVa}jJQVQVU;SMgi4e}tdFaT[l`Rjc~UQFj^UiT'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    V  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="4,5,6" nextValidID="20"><Feature recentID="3" name="30S ribosomal protein S21" directionality="2" translationMW="815.96" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6659-6679" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="2" name="rpsU" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6659-6679" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6659-7255" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7984" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7256-7260" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7690-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7282-7298" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,1,0,5,6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7309-7328" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7309-7328" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7309-7328" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7309-7328" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:15:22">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  +               .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAiY-DVf|Yi-a]Uөffedٵ)Y9fXXUo!ŽYVzHVik蚪b*InnubE&tz*IR8*ިeQiTUӞ^Rda^YE~:>Y49"cJg㪷SZGmT*ךk٦ZYDBYUYRnTQjyduIFvivfecj&XeaU'khjUeXVa}jJQVQVU;SMgi4e}tdFaT[l`Rjc~UQFj^UiT'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ    
  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="4,5,6" nextValidID="20"><Feature recentID="3" name="30S ribosomal protein S21" directionality="2" translationMW="7816.44" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6659-6859" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="2" name="rpsU" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6659-6859" color="#00e600" type="standard"/></Feature><Feature recentID="1" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6659-7435" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8164" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7436-7440" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7870-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7462-7478" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,1,0,5,6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7489-7508" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7489-7508" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7489-7508" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7489-7508" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:14:7">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  &     Q          ۛ.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAiY-DVf|Yi-a]Uөffedٵ)Y9fXXUo!ŽYVzHVik蚪b*InnubE&tz*IR8*ިeQiTUӞ^RdaPk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2<?xml version="1.0"?><Features recycledIDs="1,2,3,4,5,6" nextValidID="20"><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6658" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7387" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6659-6663" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7093-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6685-6701" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,1,0,5,6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6712-6731" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6712-6731" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6712-6731" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6712-6731" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:12:52">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  $c   
             .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features recycledIDs="0,1,2,3,4,5,6" nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6836" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6134-6150" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="2,1,0,5,6" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:11:51">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  :   	            ϛ.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAU%T5Ui'-d6ei(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBc<PklUujaQ-aUUZɃYUMUYѷZ8UFMYBTDdVh{YMyXaD(+EdKlaQ	V5"UT^	Fa5ILV:Pk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7887" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7159-7163" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="17" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7593-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="16" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="15" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7185-7201" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7158" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="5" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6369" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6369" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6625-6651" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="30S ribosomal protein S21" directionality="1" translationMW="831.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6625-6651" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q></Feature><Feature recentID="1" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6712-7158" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="hypothetical protein" directionality="1" translationMW="16767.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6712-7158" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  t<?xml version="1.0"?><Primers nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="6" name="KROL123 ∆rpsU2 FR2R" sequence="ttaagctaagttagatttcacaatagcg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7130-7157" boundStrand="1" annealedBases="ttaagctaagttagatttcacaatagcg" meltingTemperature="55"><Component hybridizedRange="7130-7157" bases="ttaagctaagttagatttcacaatagcg"/></BindingSite><BindingSite simplified="1" location="7130-7157" boundStrand="1" annealedBases="ttaagctaagttagatttcacaatagcg" meltingTemperature="55"><Component hybridizedRange="7130-7157" bases="ttaagctaagttagatttcacaatagcg"/></BindingSite></Primer><Primer recentID="5" name="KROL122 ∆rpsU2 FR1F" sequence="taaaattactaggtatcgtaaaaagtgc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6134" boundStrand="0" annealedBases="taaaattactaggtatcgtaaaaagtgc" meltingTemperature="53"><Component hybridizedRange="6107-6134" bases="taaaattactaggtatcgtaaaaagtgc"/></BindingSite><BindingSite simplified="1" location="6107-6134" boundStrand="0" annealedBases="taaaattactaggtatcgtaaaaagtgc" meltingTemperature="53"><Component hybridizedRange="6107-6134" bases="taaaattactaggtatcgtaaaaagtgc"/></BindingSite></Primer><Primer recentID="0" name="KROL121 ∆rpsU2 seq primer F" sequence="ttagaataatccactaattgaaatattgg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6531-6559" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="52"><Component hybridizedRange="6531-6559" bases="ttagaataatccactaattgaaatattgg"/></BindingSite><BindingSite simplified="1" location="6531-6559" boundStrand="0" annealedBases="ttagaataatccactaattgaaatattgg" meltingTemperature="52"><Component hybridizedRange="6531-6559" bases="ttagaataatccactaattgaaatattgg"/></BindingSite></Primer><Primer recentID="1" name="KROL119 ∆rpsU2 FR2F" sequence="atatGCGGCCGCTaaaaggtagttaaatttaattcatcgttg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6633-6670" boundStrand="0" annealedBases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttg" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6633-6670" bases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttg"/></BindingSite><BindingSite simplified="1" location="6633-6670" boundStrand="0" annealedBases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttg" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6633-6670" bases="GCGGCCGCTaaaaggtagttaaatttaattcatcgttg"/></BindingSite></Primer><Primer recentID="2" name="KROL120 ∆rpsU2 FR1R" sequence="atatGCGGCCGCgcttggcataccctctcacctcg" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6610-6640" boundStrand="1" annealedBases="GCGGCCGCgcttggcataccctctcacctcg" meltingTemperature="74"><Component bases="atat"/><Component hybridizedRange="6610-6640" bases="GCGGCCGCgcttggcataccctctcacctcg"/></BindingSite><BindingSite simplified="1" location="6610-6640" boundStrand="1" annealedBases="GCGGCCGCgcttggcataccctctcacctcg" meltingTemperature="74"><Component bases="atat"/><Component hybridizedRange="6610-6640" bases="GCGGCCGCgcttggcataccctctcacctcg"/></BindingSite></Primer><Primer recentID="3" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7212-7231" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7212-7231" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7212-7231" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7212-7231" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="4" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="14:11:40">2019.2.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .)               ƛ.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAU%T5Ui'-d6ei(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcBJVJPZV-ժ҇DVTeVF]k&fWU4haUgFBhZuVSTuچv5dNUQYgc5ad9.(D`%[`ԉUQz8$%3![B|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6625-6642" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6369" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6369" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7149" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7176-7192" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7584-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7150-7154" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7878" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7203-7222" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7203-7222" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7203-7222" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7203-7222" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:10:3">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  .W       z        z.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAU%T5Ui'-d6ei(J9ZDFfy̑cRY<WW_fVT[|FY6VtIn[`J_i:6gbeYK%rY^LBcEED_EFPY&y$ETTUU)TNDeBJVJPZV-ժ҇DVTeVF]k&fWU4haUgFBhZuVSTuچv5dNUQYgc5ad9.(D`%[`ԉUQz8$%3![B|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features recycledIDs="7,8,9,11,12" nextValidID="25"><Feature recentID="6" name="hypothetical protein" directionality="1" translationMW="16767.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6883-7329" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="5" name="FTL_1048" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6883-7329" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="30S ribosomal protein S21" directionality="1" translationMW="7840.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6625-6822" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="rpsU" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6625-6822" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="D-alanyl-D-alanine carboxypeptidase" directionality="2" translationMW="9685.55" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6108-6369" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="FTL_1046" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6369" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-7329" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7356-7372" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7764-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7330-7334" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8058" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7383-7402" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7383-7402" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7383-7402" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7383-7402" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:9:25">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  $p                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NA'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2<?xml version="1.0"?><Features recycledIDs="0,1,2,3,4,5,6,7,8,9,11,12" nextValidID="25"><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6134-6150" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6542-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6108-6112" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6836" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers recycledIDs="6,5,4,3,0" nextValidID="7"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="6161-6180" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="6161-6180" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="2" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer></Primers>
  @<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:5:0">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  B       B        B.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAW~ZjJUnڹhV;aU
^YYh0Z[>ީ.ZUi*+Ӛn:vz)OJ	TcbTkzuZ9_~kLvwPRmkN5I5im9S'JiMXj^jKE^IbFni3ij剕%|juN|NŬضN*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	        h  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7044-7060" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="18" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="14" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7452-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="13" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7018-7022" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q></Feature><Feature recentID="12" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6293-6297" color="#a6acb3" type="standard"/><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q></Feature><Feature recentID="11" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6306" maxFusedRunOn="6306"><Segment range="6293-6297" color="#993366" type="standard" translated="1"/><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q></Feature><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7746" color="#ffffff" type="standard"/><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="9" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6302-7017" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="8" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6302-6310" color="#00e600" type="standard"/></Feature><Feature recentID="7" name="proton-dependent oligopeptide transport" directionality="1" translationMW="246.24" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="512" hitsStopCodon="1"><Segment range="6302-6310" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="6" name="FTL_1252" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6307-7017" color="#00e600" type="standard"/></Feature><Feature recentID="5" name="ATP-dependent RNA helicase RhlE" 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name="transposase" directionality="1" translationMW="3618.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="193" hitsStopCodon="1"><Segment range="6108-6197" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6197" color="#00e600" type="standard"/></Feature><Feature recentID="0" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6292" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
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  A<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:4:37">2019.2.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<ꏨ*wj&mE
(4曥ljN"d'iS뒾UmbǭޝDVW=+demF5ZfMyGjfF[NTIҡ}y=6Wzi!LZbejIn,vӆpk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  j  5  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6993-7009" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="7020-7039" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7020-7039" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7020-7039" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="54"><Component hybridizedRange="7020-7039" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="2" name="KROL3 DFTL1251_FR2F" sequence="atatGCGGCCGCCaataattaatcttttgcctttggttttct" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6240-6278" boundStrand="0" annealedBases="tGCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="6240-6278" bases="tGCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite><BindingSite simplified="1" location="6240-6278" boundStrand="0" annealedBases="tGCGGCCGCCaataattaatcttttgcctttggttttct" meltingTemperature="67"><Component bases="ata"/><Component hybridizedRange="6240-6278" bases="tGCGGCCGCCaataattaatcttttgcctttggttttct"/></BindingSite></Primer><Primer recentID="3" name="KROL4 DFTL1251_FR2R" sequence="atatGGTACCgtatcagctgatgttgtaaatacgac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6940-6971" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6940-6971" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite><BindingSite simplified="1" location="6940-6971" boundStrand="1" annealedBases="GGTACCgtatcagctgatgttgtaaatacgac" meltingTemperature="61"><Component bases="atat"/><Component hybridizedRange="6940-6971" bases="GGTACCgtatcagctgatgttgtaaatacgac"/></BindingSite></Primer><Primer recentID="4" name="KROL1 DFTL1251_FR1F" sequence="atatGGATCCttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6101-6137" boundStrand="0" annealedBases="GGATCCttagttttcaaaaatataatctttgctattc" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="6101-6137" bases="GGATCCttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6101-6137" boundStrand="0" annealedBases="GGATCCttagttttcaaaaatataatctttgctattc" meltingTemperature="57"><Component bases="atat"/><Component hybridizedRange="6101-6137" bases="GGATCCttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="5" name="KROL2 DFTL1251_FR1R" sequence="atatGCGGCCGCaatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6211-6248" boundStrand="1" annealedBases="GCGGCCGCaatagtcacaattattctcctattttaaac" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6211-6248" bases="GCGGCCGCaatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6211-6248" boundStrand="1" annealedBases="GCGGCCGCaatagtcacaattattctcctattttaaac" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="6211-6248" bases="GCGGCCGCaatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="16:10:19">2018.10.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  1       C        C.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #*<?xml version="1.0"?><Features recycledIDs="11,12,13,14" nextValidID="25"><Feature recentID="24" name="proton-dependent oligopeptide transport" directionality="1" translationMW="331.43" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6283" maxFusedRunOn="6283"><Segment range="6233-6241" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="23" name="FTL_1251" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6233-6241" color="#00e600" type="standard"/></Feature><Feature recentID="22" name="transposase" directionality="1" translationMW="1461.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="210" hitsStopCodon="1"><Segment range="6108-6146" color="#ff0000" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="18" name="isftu2" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6146" color="#00e600" type="standard"/></Feature><Feature recentID="17" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6108-6241" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6979" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translationMW="75.07" type="CDS" allowSegmentOverlaps="0" readingFrame="3" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="6354" maxFusedRunOn="6354"><Segment range="6242-6246" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6242-6246" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="117.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6214" maxFusedRunOn="6214"><Segment range="6251-6255" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6251-6255" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6685-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6277-6293" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="KROL1 DFTL1251_FR1F" sequence="ttagttttcaaaaatataatctttgctattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite><BindingSite simplified="1" location="6107-6137" boundStrand="0" annealedBases="ttagttttcaaaaatataatctttgctattc" meltingTemperature="51"><Component hybridizedRange="6107-6137" bases="ttagttttcaaaaatataatctttgctattc"/></BindingSite></Primer><Primer recentID="0" name="KROL2 DFTL1251_FR1R" sequence="aatagtcacaattattctcctattttaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite><BindingSite simplified="1" location="6211-6240" boundStrand="1" annealedBases="aatagtcacaattattctcctattttaaac" meltingTemperature="53"><Component hybridizedRange="6211-6240" bases="aatagtcacaattattctcctattttaaac"/></BindingSite></Primer></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:35:2">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ;       ;        ;.	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAUf[涮Z**j~UBhVAZ!<:hkYz]Tj:U{ZUnkfiTjJDQNNIj=hrH`iQffiښWݚdڄ iUUE[ign֡%6YYi[SYFid(dij數暊*)Vd]WZaayYZeRjYVeVijtiiiV&Yi?[9VmugkoVI[jVfʎԋ7o$e@k%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>    `  j  a  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature 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  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:34:17">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  1                .	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAϣ	XUFnYV)jiifJ^En։g*DMnhiv}e}٦II%X	UZd[hyZ?Fz
j[]QYnjEEU5JDfIbHZibnVIYUۍzoIUNVuU&fZ5ijjGJVeijfY=^Xv}fdeflHq&BJYji]4	;[b8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6122-6130" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6402" 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swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7567-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7159-7175" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="21:34:13">2018.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  6        "        ".	Gq4]D&$0tJR>^py]&8SҟQ($	0SMs4UTon^ܥamU~FiU*Rfa%z[P(*3ktQhh;ֺ<:,Bý04u	0ϝoc6V!8%LoN/n~>M\<Qm P2NUif)9*ZT[:*U]UZj{YDFխV:WUeuA%)k\rCiZᩘ	X5#nlh?D5BLFx8pZmMo'c`1UkКEo!Vh+<(ƾ*%2j˳3[qae HjLRTV6W=!骇id(q,!*i\:Uf	fV*چ;Jh)h(x4M8z:Vѷ64| 5R[}x/~u;546pa6F>2ZeyiSiM
ha0gfbjDVWV@+ݫU#߇.Q_ivUd2m?.ʰ!WӿpNLGS3-0",0DѢxD[ꌪ^)htb43W(hfNmh΄'LR!%%BmF]=*EWe6%fG%-lџj<Tr2n))
ު*N<<ΣE*pQad)YQ$J􊎵g>뜒n2`τJ>'jr-Q+TN.ᜥ(%V$Y\F%b"ڍj,TsL8JǪEؕj9cTq$*1<ʢX=8W/CFjfڪ+@j4ٶfXb`ꂲ΄>0T)g:*^.bxL.:FJJsuUf% =Rgzwj>:Iu3{hׂdUUGV⮫kd6eJ>[uffyYdBf!TNDMlm|#0,J0=N/qj?M	>lPwd 8
n)$4M(MЌY@t;0 >zߛ(PT_V1UgUa)0U3*n|xՍix@DqTNwĀoS<2C(0!Ytc%U6͚Y]TJUo-:Z>*`:ro;{A+|uuѕqP.xO*>+̀wl32넬6  |xծ*Gθn&Vo=WOSkC׊@0>9=81PPƔ%@-(q=NAbfV9jefId)jfbeIilߖV*_J^Yx4mM2JRfD}rM%MĩnjnQO6mZ7זUzwYHdfJH$c֪٨jQmhA4gXeJ%ͦ>NWWYIeRFjE6Jg^ڢ%qJaiNa^NvvV_c*Ine.ꑎej훦 &&Zh'j,QUdYa)]deX9>&-cV^UNf^֦[ZjٚDU)zSZOZ%+i6Yif&&j`&UimhjAh꛸*uEmuFIfMmZjVyTy)Y%"ZnڪjyUY%eWn6ꩽ%V:eXkEVTUjdեZje*ZcEZiIZOg]dyaՒaڕ"ě	+e=;Yt'Zmd⯈hZuSeH> ih3ίK_#Ӎ*2h;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ          !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-8888" color="#ffffff" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q><Q name="note"><V text="/dnas_title=pKL80 pEX.DFTL_0880"/></Q></Feature><Feature recentID="20" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="341.40" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7131-7139" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="19" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7131-7139" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="18" name="FTL_0879" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6097" maxFusedRunOn="6097"><Segment range="6113-6702" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256237" predef="GI"/><V text="3952353" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513599.1"/></Q><Q name="inference"><V bool="1" text="2004" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="beta-lactamase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q><Q name="note"><V text="/dnas_title=beta-lactamase"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q></Feature><Feature recentID="17" name="FTL_0879" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6113-6702" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952353" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0879"/></Q></Feature><Feature recentID="16" name="FTL_0882" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7943" maxFusedRunOn="7943"><Segment range="7974-8164" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256240" predef="GI"/><V text="3952356" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513602.1"/></Q><Q name="inference"><V bool="1" text="4.1" predef="profile"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="apolipoprotein N-acyltransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q><Q name="note"><V text="/dnas_title=apolipoprotein N-acyltransferase"/></Q></Feature><Feature recentID="15" name="FTL_0882" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7974-8164" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952356" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0882"/></Q></Feature><Feature recentID="14" name="FTL_0880" directionality="1" translateFirstCodonAsMet="1" translationMW="319.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7238" maxFusedRunOn="7238"><Segment range="7149-7157" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256238" predef="GI"/><V text="3952354" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513600.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="13" name="FTL_0880" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7149-7157" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952354" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0880"/></Q></Feature><Feature recentID="12" name="FTL_0881" directionality="2" translateFirstCodonAsMet="1" translationMW="-1.00" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7429" maxFusedRunOn="7429"><Segment range="7453-7728" color="#993366" type="standard" translated="1"/><Q name="db_xref"><V text="89256239" predef="GI"/><V text="3952355" predef="GeneID"/></Q><Q name="protein_id"><V text="YP_513601.1"/></Q><Q name="inference"><V bool="1" text="Uniprot" predef="similar to AA sequence"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="hypothetical protein"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q><Q name="note"><V text="/dnas_title=hypothetical protein"/></Q></Feature><Feature recentID="11" name="FTL_0881" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7453-7728" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="3952355" predef="GeneID"/></Q><Q name="locus_tag"><V text="FTL_0881"/></Q></Feature><Feature recentID="10" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8594-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="9" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="8" name="SacB" directionality="2" translationMW="52972.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8186-8202" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  C<Notes>
<UUID>265140f3-4473-461e-a6f2-0808c24fa2b0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:34:38">2018.2.27</Created>
<LastModified UTC="23:40:14">2018.2.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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}*aR#08W" Dm4GăE5PUZ|~7qoNҕ|t{Cd]9oXO=8B:o&c.E\[J:yFՂ	l)QEfd>Ɠ.yA꼇T16/?;TO{3,ZMXOC)uK%zBxl1ldv0:+n-Dg1L_q+}gUj>>A-WyU(:̇lnZ"c߽%JJGp9YF˩$'5maJZ\    iIAO # :g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="0" name="ColE origin" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7697-288" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="ColE origin"/></Q></Feature><Feature recentID="1" name="promoter" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="866-1103" color="#ffffff" type="standard"/><Q name="note"><V text="groES promotoer"/></Q></Feature><Feature recentID="2" name="SacB" directionality="2" translationMW="52971.10" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4038" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2704-4038" color="#993366" type="standard" translated="1"/><Segment name="signal peptide" range="4039-4125" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Bacillus subtilis sacB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;negative selection marker&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;secreted levansucrase that renders bacterial growth sensitive to sucrose&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"/></Q></Feature><Feature recentID="3" name="KanR" directionality="1" translationMW="30979.26" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1104-1919" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="4" name="sacB promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4126-4571" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacR&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;sacB&lt;/i&gt; promoter and control region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="oriT" type="oriT" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4945-5054" color="#a6acb3" type="standard"/><Q name="note"><V text="incP origin of transfer"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2296-2400" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="rrnB T1 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5616-5702" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Escherichia coli rrnB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T1 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="rrnB T2 terminator" type="terminator" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5497-5524" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator T2 from the &lt;i&gt;E. coli rrnB&lt;/i&gt; gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6052-6068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7289-7305" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pyrE" directionality="2" type="gene" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6108-6448" color="#00e600" type="standard"/></Feature><Feature recentID="12" name="orotate phosphoribosyltransferase" directionality="2" translationMW="12544.57" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5858" maxFusedRunOn="5858"><Segment range="6108-6448" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MFIEFALKNQVLKFGEFTLKSGRISPYFFNAGLFNTGTQLATLADYYAQLIIKSNVKYDILFGPAYKGIPLVAAISTVLALKYNIDMPYAFDRKEAKDHGEGGVFVGADMTNK"/></Q></Feature><Feature recentID="13" name="FTL_0508" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6608-6616" color="#00e600" type="standard"/></Feature><Feature recentID="14" name="UDP-N-acetylmuramate" directionality="1" translationMW="364.47" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6634" maxFusedRunOn="6634"><Segment range="6608-6616" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSK"/></Q></Feature><Feature recentID="15" name="FTL_0508" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6626-6634" color="#00e600" type="standard"/></Feature><Feature recentID="16" name="UDP-N-acetylmuramate" directionality="1" translationMW="259.35" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="455" hitsStopCodon="1"><Segment range="6626-6634" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IK*"/></Q></Feature><Feature recentID="17" name="FTL_0509" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6631-7262" color="#00e600" type="standard"/></Feature><Feature recentID="18" name="tRNA/rRNA methyltransferase" directionality="1" translationMW="22800.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7284" maxFusedRunOn="7284"><Segment range="6631-7262" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSSLIYGIHAVDSLVEANQAKEILVFKKSNANHKIEGIISKAESKGLKVTFIDSFKQLPGRVRKDANHQNIFAVEINDFKTYSENDIEFLVPQDKNAFILILDNVQDPHNFGACIRSAHSAGIDFIIIPKDNSAPVNATVKKVACGAAEHTKIVVVTNLARAIEKLKQLGVWIIGLAGEADDSLYSMNLVDSVAIVAGAEGSGMRQRTKA"/></Q></Feature></Features>
  Y<?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="KROL7 Seq_pEX_2" sequence="TGTGGAATTGTGAGCGGATA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite location="7316-7335" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7316-7335" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite location="7566-7576" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="7571-7576" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="7566-7569" bases="GATA"/></BindingSite><BindingSite simplified="1" location="2345-2354" boundStrand="0" annealedBases="TGAGCGGATA" meltingTemperature="27"><Component bases="TGTGGAATTG"/><Component hybridizedRange="2345-2354" bases="TGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7316-7335" boundStrand="1" annealedBases="TGTGGAATTGTGAGCGGATA" meltingTemperature="55"><Component hybridizedRange="7316-7335" bases="TGTGGAATTGTGAGCGGATA"/></BindingSite><BindingSite simplified="1" location="7566-7576" boundStrand="1" annealedBases="GTGAGCGATA" meltingTemperature="21"><Component bases="TGTGGAATT"/><Component hybridizedRange="7571-7576" bases="GTGAGC"/><Component bases="G"/><Component hybridizedRange="7566-7569" bases="GATA"/></BindingSite></Primer><Primer recentID="1" name="KROL6 Seq_pEX_1" sequence="GTTTTCCCAGTCACGACGTT" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite><BindingSite simplified="1" location="6031-6050" boundStrand="0" annealedBases="GTTTTCCCAGTCACGACGTT" meltingTemperature="57"><Component hybridizedRange="6031-6050" bases="GTTTTCCCAGTCACGACGTT"/></BindingSite></Primer><Primer recentID="2" name="KROL660 delt_FTL0508_FR1_R" sequence="atatGCGGCCGCttttgacattaaaaactttttaaa" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-08T17:59:53Z"><BindingSite location="6592-6624" boundStrand="1" annealedBases="tGCGGCCGCttttgacattaaaaactttttaaa" meltingTemperature="63"><Component bases="ata"/><Component hybridizedRange="6592-6624" bases="tGCGGCCGCttttgacattaaaaactttttaaa"/></BindingSite><BindingSite simplified="1" location="6592-6624" boundStrand="1" annealedBases="tGCGGCCGCttttgacattaaaaactttttaaa" meltingTemperature="63"><Component bases="ata"/><Component hybridizedRange="6592-6624" bases="tGCGGCCGCttttgacattaaaaactttttaaa"/></BindingSite></Primer><Primer recentID="3" name="KROL661 delt_FTL0508_FR1_F" sequence="atatGGATCCtttttattagtcatatcagcaccaacaaat" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-08T17:59:53Z"><BindingSite location="6101-6136" boundStrand="0" annealedBases="GGATCCtttttattagtcatatcagcaccaacaaat" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6136" bases="GGATCCtttttattagtcatatcagcaccaacaaat"/></BindingSite><BindingSite simplified="1" location="6101-6136" boundStrand="0" annealedBases="GGATCCtttttattagtcatatcagcaccaacaaat" meltingTemperature="60"><Component bases="atat"/><Component hybridizedRange="6101-6136" bases="GGATCCtttttattagtcatatcagcaccaacaaat"/></BindingSite></Primer><Primer recentID="4" name="KROL662 delt_FTL0508_FR2_F" sequence="atatGCGGCCGCAattaaatgagtagtttaatttatggtattc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-08T18:00:41Z"><BindingSite location="6616-6654" boundStrand="0" annealedBases="GCGGCCGCAattaaatgagtagtttaatttatggtattc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6616-6654" bases="GCGGCCGCAattaaatgagtagtttaatttatggtattc"/></BindingSite><BindingSite simplified="1" location="6616-6654" boundStrand="0" annealedBases="GCGGCCGCAattaaatgagtagtttaatttatggtattc" meltingTemperature="62"><Component bases="atat"/><Component hybridizedRange="6616-6654" bases="GCGGCCGCAattaaatgagtagtttaatttatggtattc"/></BindingSite></Primer><Primer recentID="5" name="KROL663 delt_FTL0508_FR2_R" sequence="atatGGTACCatagcttttgtacgctgacgcatac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-05-08T18:00:42Z"><BindingSite location="7237-7267" boundStrand="1" annealedBases="GGTACCatagcttttgtacgctgacgcatac" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="7237-7267" bases="GGTACCatagcttttgtacgctgacgcatac"/></BindingSite><BindingSite simplified="1" location="7237-7267" boundStrand="1" annealedBases="GGTACCatagcttttgtacgctgacgcatac" meltingTemperature="64"><Component bases="atat"/><Component hybridizedRange="7237-7267" bases="GGTACCatagcttttgtacgctgacgcatac"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>85d84aa4-587c-4203-9f83-f86139f33e68</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="18:3:7">2023.5.8</Created>
<LastModified UTC="18:3:7">2023.5.8</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         Unique 6+ Cutters                                                                                           d     CHR  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
