	   SnapGene      patatGAATTCatgtcaaaacatattcatattttaggtatctgtggtacttttatgggttctttagcagtattagctaaacaaaagggatataaagtaacaggttcggatcttaatgtctatccaccaatgagtacttatctcgagtctcaaggcatagagatattacaaggatttgattgtgatcaactagatacaaatcctgatgaaattattattggtaatatcatgaaaagaggtatgccaataatagaaaaaatcctcgcagaaaagttaaactatttctcaggtccagagtggctatatcaaaatattcttaaatataaaaaagtaattgcaatagctggtacacatggtaaaactacaaccactacaatgactatcaaaatactagagcaggctggcttaaatccgagttttttggttggtggtgttagtagtgattttggtgtttcatcacgttacacagattctgagtattttgttattgaagctgatgaatatgatacggctttttttgataagcgttcaaaattgattcattatgatccaagtatttttgtgattaacaatattgaatttgatcatgctgatattttcaaagacattgatgcaatattttggcaatttcaccaattacttagaaagatgccatcaacagcaaagattatctacaacgctaagaatgataatgtacaaaaaataatatcaatgggatgttggtcagagttggttaaagtaaattctgatttaggtatcagtataaccaagcacacactagattactcaaagtttgaattatgtgatattaatggtaatagtgttgaagtatcatggggcttaattggtgagcataatgctcttaatgctatgagtgcctatgctgtagcaaagcaactaaatatatctgatgaaatggtaaaagacgctttagaaagctttagaggagttaaaagacgtttagaagtattatctcatcaagataatgttactttatatgatgattttgctcatcatccaacctctattaagttaactttagaagcagtgcgtaacaaagctaaagatgcttatgtagtagctcttattgacccacgttcaaatacaatgcgtcaaggcgataataaagataatttaccgatgtcaattatcgaagctgatagagtattgttgtataatcatagtttattaaagtgggatgctaaagaagttctcaaaaatagtaataatgttgattttatagctgatgttgatgattttgttgattgtgttgataaattattaactaagcaccaagatagaaatatccagcttgtaatgatgagtaatggctcgtttgatggattaagagaaaagctagttaagcttttggagattaaatgaGGATCCatat                                  	                            
         	                                                          	   	                                                                                         
               	         	                                                                
   
   	                                                    
             	                                    	                                                         
               	         	            
                                                                                                                                                                                                                        	                                                                                    A T  6  B X   U i  ;e   L	 " +   W`+ 7 d   Z  l (C/    c  BY
   $f( O 7~	    	D  >    _'q%8 V   M"]f   1
<[ j   Df  Y w ? ) &  / M 2 h s03 ^  Ha, * # C  ': ,  X	 4	 M 4 8 e F    Ut s      [ 
S   @  Nbmv{  Q   TJp I )a!I D*R X60     j   S   d ! c    Y   #A
  C   	&eZ X %1g B ]  G&92)	 L   		 ? Puz H S 5HQ   R   X  W%d@ \ F%8 yGP Q [  EVx$cF  P     
1BCDc~E    2<J_  L d p ~ t(U^P",`    3=P8d.K`   J?oxHiw      M ` 5  e} k 4 ^)?-9     ud#&a
 
 q .r
  H`V_n(Q	   Q/ Y  @S^ag     W   ,4>9Ne#k  L   K;\I< E    2y% x=\   )@Pgpj   6 {   .{E< % k   w   !g    D  a 
 N   ir6N  \Z Z  	  fox~ .  |	  n { lu	  @ 5 @ :W *  V_*K 'k.  Ne'b	    =6    0CL^!$7 v> (  . r/ 3  r s R Ma IW`o)  i        01K > G Rt4 ?O K Z ;Ab#b^ o   I'-GT]_h!+ _ 4 
  3  cm- X +?   D    5(:Of$; $ v   -z;z 	   YhqM -  { m	  jJV  <B]5=6  - L
  = F ~ n :3&F\>N] 2    ,:y  zl  ,Ai5U  1 < k  0  *+QhT Rq f    SrG 9 7   g   | 2      /|THVu    F= s &  t  : l 8Z8     x  "h       h       } \p      E3 T       b  " A     $W  O  0}UwI }  Os7cnwOv  j| 	  ]G>    Rj [  C [ n z t U  K ' q    J J " u *ybl gp 	 m  9}E[+9 / ; 
S    1   Y7     o     ! |  #v
 i y mB   k      A        GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  4E  4g  4g  4i  4  5=  6_  = =   @ Xa!I ng   > %e Cqe  i e PX       38d 'i e f<S5G Ls8_'}S g ^       ,  XH S 5HQ Jj   Df	  ]G>H (SC 5/HQ 	   3 X    +?]G>Rcm-jDMa !b U  J?oxHiw cn :3&F\>N]! H c  S 5DHQ |  l7zXZ  ִF !   t/	)] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!ǚ^!8?>H=}'^|<\rL #Go{!g͈o߶}DVlr}nE9EpJPʄ5Oֽ7~(3|hXL>8w
Q&ãJU><5FtcqL4 X~%\q2H,hSeAɆA/v䗉*b>!PD@OtAލ!mN%k0Ե8ӱ_\J0maDǄxT>~ېr%|S==T.UʰX*.cR$a\0j[
qj$Sc#aL%PUG8.]|v"-Bc2F	!O; ]bBaF.&`v'i +uhȼ%D/qU.xNm'
2[ܺS`q,hp28dik	lM9Z
=oGW[*nO.>,h\YfW%"nI9Pę\L3TLx2ʳb>!RS`{5b-dI(n˦1K9\Cz})6InVH֙;%.!o/d(t:YF\fQU͆eʞMbyO {e9/[63zcDc 0嶽AZ[     
_{|6   *E7g    YZ   -<AlignableSequences trimStringency="Medium"/>  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="5"><Feature recentID="0" name="tRNA/rRNA methyltransferase" directionality="1" translationMW="149.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1391" maxFusedRunOn="1389"><Segment range="1378-1382" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M"/></Q></Feature><Feature recentID="1" name="FTL_0509" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1378-1382" color="#00e600" type="standard"/></Feature><Feature recentID="2" name="UDP-N-acetylmuramate" directionality="1" translationMW="51528.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="11-1381" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQLDTNPDEIIIGNIMKRGMPIIEKILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTTTMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIEADEYDTAFFDKRSKLIHYDPSIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSELVKVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISDEMVKDALESFRGVKRRLEVLSHQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALIDPRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEVLKNSNNVDFIADVDDFVDCVDKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLEIK*"/></Q></Feature><Feature recentID="3" name="FTL_0508" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-1381" color="#00e600" type="standard"/></Feature><Feature recentID="4" name="Francisella tularensis subsp. holarctica" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11-1382" color="#000000" type="standard"/><Q name="organism"><V text="synthetic DNA construct"/></Q></Feature></Features>
  
U<?xml version="1.0"?><Primers nextValidID="3"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="P433 FTL0702cmp_R2" sequence="atatGGATCCtcattcttctttaggcaaaacattactt" description="Contains BamHI site"><BindingSite location="1376-1390" boundStrand="1" annealedBases="atatGGATCCtcatt" meltingTemperature="40"><Component hybridizedRange="1376-1390" bases="atatGGATCCtcatt"/><Component bases="cttctttaggcaaaacattactt"/></BindingSite><BindingSite simplified="1" location="1376-1390" boundStrand="1" annealedBases="atatGGATCCtcatt" meltingTemperature="40"><Component hybridizedRange="1376-1390" bases="atatGGATCCtcatt"/><Component bases="cttctttaggcaaaacattactt"/></BindingSite></Primer><Primer recentID="1" name="KROL670 FTL_0508_EcoRI_F" sequence="atatGAATTCatgtcaaaacatattcatattttaggtatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-06-02T13:54:20Z"><BindingSite location="0-39" boundStrand="0" annealedBases="atatGAATTCatgtcaaaacatattcatattttaggtatc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGAATTCatgtcaaaacatattcatattttaggtatc"/></BindingSite><BindingSite location="747-756" boundStrand="0" annealedBases="tttaggtatc" meltingTemperature="20"><Component bases="atatGAATTCatgtcaaaacatattcatat"/><Component hybridizedRange="747-756" bases="tttaggtatc"/></BindingSite><BindingSite simplified="1" location="0-39" boundStrand="0" annealedBases="atatGAATTCatgtcaaaacatattcatattttaggtatc" meltingTemperature="57"><Component hybridizedRange="0-39" bases="atatGAATTCatgtcaaaacatattcatattttaggtatc"/></BindingSite><BindingSite simplified="1" location="747-756" boundStrand="0" annealedBases="tttaggtatc" meltingTemperature="20"><Component bases="atatGAATTCatgtcaaaacatattcatat"/><Component hybridizedRange="747-756" bases="tttaggtatc"/></BindingSite></Primer><Primer recentID="2" name="KROL671 FTL_0508_BamHI_R" sequence="atatGGATCCtcatttaatctccaaaagcttaactagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2023-06-02T13:54:21Z"><BindingSite location="1353-1390" boundStrand="1" annealedBases="atatGGATCCtcatttaatctccaaaagcttaactagc" meltingTemperature="61"><Component hybridizedRange="1353-1390" bases="atatGGATCCtcatttaatctccaaaagcttaactagc"/></BindingSite><BindingSite simplified="1" location="1353-1390" boundStrand="1" annealedBases="atatGGATCCtcatttaatctccaaaagcttaactagc" meltingTemperature="61"><Component hybridizedRange="1353-1390" bases="atatGGATCCtcatttaatctccaaaagcttaactagc"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>464ca59a-ae33-4eb8-902c-c6f5c043ae79</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="13:54:24">2023.6.2</Created>
<LastModified UTC="13:54:24">2023.6.2</LastModified>
<CreatedBy>kramseylab</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         6+ Cutters                                                                                                  d     CHO  d              E             Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
